狭鳕鱼皮胶原蛋白肽对大鼠关节炎模型中氧化应激和炎症因子的影响  

Effect of Collagen Peptides from Walleye Pollock Skin on Oxidative Stress and Inflammatory Cytokine in Osteoarthritis Rats

作　　者：孔德新[1] 陈雪红[1] 谢靖[1] 王春波[1] 韩彦弢[1] 

机构地区：[1]青岛大学药学院,山东青岛266071

出　　处：《安徽农业科学》2015年第26期132-134,共3页Journal of Anhui Agricultural Sciences

基　　金：青岛市科技局项目(14-2-3-50-nsh)

摘　　要：[目的]建立大鼠关节炎模型,探讨狭鳕鱼皮胶原蛋白肽对大鼠关节炎模型的抗氧化和抗炎机理。[方法]采用切断左侧髌韧带及跟腱法建立大鼠关节炎模型;治疗组给予不同剂量的狭鳕鱼皮胶原蛋白肽灌胃;采用HE染色观察大鼠关节软骨的组织病理学变化;采用酶联免疫法检测大鼠血清中氧化损伤相关指标丙二醛(MDA)、一氧化氮(NO)、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)的含量;采用ELISA法测定大鼠血清炎症因子肿瘤坏死因子α(TNF-α)和白介素-1(IL-1)的含量。[结果]狭鳕鱼皮预防组、狭鳕鱼皮剂量Ⅰ组、狭鳕鱼皮剂量Ⅱ组关节软骨的损伤得到改善,骨关节炎大鼠血清中的MDA、NO的含量明显降低,SOD和T-AOC的活性有所提高,与模型组相比差异极显著(P<0.01)。各剂量狭鳕鱼皮组大鼠血清中TNF-α和IL-1的含量都有所降低。[结论]狭鳕鱼皮胶原蛋白肽可以有效缓解大鼠骨关节炎的症状,其机制可能与调控体内的氧化-抗氧化的平衡体系以及抑制炎症因子的释放有关。[Objective]To establish the model of osteoarthritis rats and investigate the protective effect of collagen peptides from walleye pollock skin and its possible mechanisms concerning antioxidant and anti-inflammation. [Method] Cutting off the left side of the patellar ligament and tendon was used to establish the model of osteoarthritis rats; The treatment groups were intragastric administration by different doses of collagen peptides from walleye pollock skin; Enzyme biochemical method was used to detect the levels of MDA,NO,SOD and T-AOC in rats serum;El ISA was applied to detect the levels of inflammatory cytokine TNF-α and IL-1. [Result]All treatment groups of collagen peptides from walleye pollock skin could reduce the levels of MDA and NO and improve the content of SOD and T-AOC in the rats serum. Compared with model group,the difference was extremely significant（ P 〈0. 01）. Moreover,all treatment groups could reduce the levels of TNF-α and IL-1 in rats serum.[Conclusion]The collagen peptides from walleye pollock skin could effectively alleviate the symptoms of osteoarthritis in rats and its mechanism may be related to maintain the balance between oxidant and antioxidant system as well as inhibit the release of inflammatory cytokine.

关 键 词：狭鳕鱼皮胶原蛋白 关节炎 氧化应激 炎症因子 

分 类 号：S865.1[农业科学—野生动物驯养]