3个鹌鹑群体的微卫星标记多态性分析  

Polymorphism Analysis of 3 Quail Groups by Using Microsatellite Marker

作　　者：白俊艳[1] 庞有志[1] 张小辉[1] 贠银现[1] 赵淑娟[1] 祁艳霞[1] 

机构地区：[1]河南科技大学动物科技学院,洛阳471003

出　　处：《中国畜牧兽医》2015年第9期2444-2452,共9页China Animal Husbandry & Veterinary Medicine

基　　金：河南省重点科技攻关项目(082102130002)

摘　　要：为了从分子水平上揭示朝鲜鹌鹑、中国黄羽鹌鹑、中国黑羽鹌鹑3个鹌鹑群体的遗传结构和亲缘关系,为鹌鹑的遗传资源研究提供科学依据,本研究采用PCR扩增、聚丙烯酰胺凝胶电泳、聚类分析等方法对3个鹌鹑群体进行了微卫星标记的多态性分析。结果表明,12个微卫星标记在3个鹌鹑群体中检测到的观测等位基因数在4~7个之间,中国黄羽鹌鹑、中国黑羽鹌鹑、朝鲜鹌鹑的平均多态信息含量分别为0.6853、0.6401和0.6565;平均期望杂合度分别为0.7333、0.6957和0.7111,以中国黄羽鹌鹑群体遗传多态样性最为丰富。聚类分析结果表明,中国黑羽鹌鹑和朝鲜鹌鹑的遗传距离最小为0.0628,因此中国黑羽鹌鹑和朝鲜鹌鹑先聚为一类,而后与中国黄羽鹌鹑聚合在一起。这也更进一步证明了中国黑羽鹌鹑和朝鲜鹌鹑有更近的亲缘关系,而中国黑羽鹌鹑,朝鲜鹌鹑与中国黄羽鹌鹑的亲缘关系要远一些。In order to reveal the genetic structure and genetic relationship of Korean quail,Chinese Yellow quail,Chinese Black quail,the 3quail populations from the molecular level,to provide for the study of genetic resources of quail.This study used PCR amplification,polyacrylamide gel electrophoresis,cluster analysis and other methods of 3quail populations were polymorphic analysis of microsatellite markers.The results showed that 12 microsatellite markers in 3quails was detected in the observed number of alleles was between 4and 7,the average polymorphism information content of Chinese Yellow quail,Chinese Black quail,Korean quail were 0.6853,0.6401 and 0.6565,respectively;The average heterozygosity were 0.7333,0.6957 and 0.7111,respectively,which Chinese Yellow quail population genetic diversity was the most abundant polymorphism.The cluster analysis showed that the Chinese Black quail and Korean quail smallest genetic distance was 0.0628,so the Chinese Black quail and Korean quail were clustered into one category,and then was Chinese Yellow quail together.This was further evidence of Chinese Black quail and Korean quail had a closer genetic relationship,and the genetic relationship of Chinese Black quail and Korean quail was more distant with Chinese Yellow quail.

关 键 词：鹌鹑 微卫星标记 多态性 遗传多样性 

分 类 号：S839.2[农业科学—畜牧学]