氟暴露儿童ERα基因甲基化水平与氟斑牙及血清钙水平的关系  

Relationship between dental fluorosis,serum calcium level,and ERαgene methylation in children exposed to fluoride

作　　者：王科妍 崔蕊蕊[2] 丁中[3] 崔留欣[1] 赵明旭[1] 程学敏[1] 巴月[1] 

机构地区：[1]郑州大学公共卫生学院环境卫生学教研室,郑州450001 [2]山东万杰医学院山东省高校生物医学工程技术实验室,淄博255213 [3]开封市疾病预防控制中心地方病科,开封475000

出　　处：《郑州大学学报（医学版）》2015年第5期627-630,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81072247;河南省教育厅科学技术研究重点项目13A330653

摘　　要：目的：分析雌激素受体α（ ERα）基因启动子区甲基化水平与儿童氟斑牙及血清钙水平的关系。方法：采用整群抽样的方法抽取河南省某县氟病区2所小学8～12岁儿童174人，采用Dean分级法对儿童氟斑牙进行分级，并依据病情分为正常组（n＝85）、轻度组（n＝53）和中重度组（n＝36）。采用原子吸收分光光度法测定血清钙含量，采用氟离子选择电极法测定尿氟含量，采用real-time PCR法检测ERα基因启动子区甲基化率。结果：中重度组男性儿童ERα基因启动子区甲基化率为（2．61±0．95）％，高于正常组儿童的（1．89±0．74）％和轻度组儿童的（1．99±0．89）％（P均＜0．05）；3组女性儿童ERα基因启动子区甲基化率比较，差异无统计学意义（F＝0．585， P＝0．559）。 ERα基因启动子区甲基化率与血清钙水平呈负相关（r＝－0．172，P＝0．024）。结论：ERα基因启动子区甲基化水平可能与儿童氟斑牙患病程度有关，且存在性别差异；ERα基因启动子区甲基化水平与血清钙水平呈负相关。Aim：To explore the relationship between estrogen receptor α（ ERα） gene methylation and children dental fluorosis,serum calcium level .Methods：A total of 174 primary school students aged 8~12 years, born and raised up in fluoride exposure areas of Henan Province were selected by cluster sampling , and were divided into normal group（n=85）, mild group（n=53）, as well as moderate and severe group （n=36） according to dental fluorosis degree .Dental fluorosis was identified with Dean method .Urine fluoride and serum calcium levels were determined using fluoride ion selective elec -trode and flame atomic absorption spectrometry methods , respectively .The methylation level of ERαgene promoter was de-tected by real-time PCR procedure .Results：The percentage of ERαgene methylation in the boys of moderate and severe group[（2.61 ±0.95）%] was significantly higher than those in normal group [（1.89 ±0.74）%] and mild group [（1.99 ±0.89）%]（P〈0.05）.No significant difference in ERαgene methylation percentage was observed among the girls from different groups（F=0.585,P=0.559）.Negative correlation between methylation level in ERαgene and serum＆amp;nbsp;calcium level of children was observed （r=-0.172,P=0.024）.Conclusion：ERαgene methylation may be related with the degree of children dental fluorosis and this may have gender specificity .There is a negative correlation between the methylation level of ERαgene and serum calcium level .

关 键 词：氟斑牙 雌激素受体Α DNA甲基化 血清钙 

分 类 号：R599[医药卫生—内科学]