慢性轻度不可预见性应激大鼠血液组分中17种氧化应激相关元素的测定分析  

Measurement and analysis of oxidative stress-related elements in blood components of chronic unpredictable mild stress rats

作　　者：文华[1] 刘多见[1] 王栋芳[1] 刘雅琼[1] 闫赖赖[1] 曾静[1] 卢庆彬[1] 欧阳荔[1] 王京宇[1] 

机构地区：[1]北京大学公共卫生学院,北京100191

出　　处：《卫生研究》2015年第5期806-812,共7页Journal of Hygiene Research

摘　　要：目的研究慢性轻度不可预见性应激(CUMS)对大鼠血浆和红细胞中17种氧化应激有关化学元素含量及分布的影响。方法 40只大鼠随机分为应激组和对照组,应激组使用CUMS方法处理28 d后测定其血浆、红细胞中17种化学元素的含量,分析元素间相关性。结果应激组血浆钼、硼元素及红细胞硒元素浓度高于对照组(P<0.05);血浆锡、铬、钍、铊元素及红细胞锰、铅元素浓度低于对照组(P<0.05)。元素间相关强度及方向改变:血浆钍-铁在对照组负相关(r=-0.581,P=0.018),在应激组正相关(r=0.473,P=0.035);血浆钍-镁在对照组负相关(r=-0.610,P=0.012),在应激组正相关(r=0.596,P=0.006)。结论 CUMS改变大鼠血浆及红细胞中多种氧化应激相关元素的含量及元素间相关性分布,并且含量升高的元素均为抗氧化应激元素,降低的元素多为促氧化应激元素。Objective To study the effects of chronic unpredictable mild stress（ CUMS） on the concentrations and distribution of elements related to oxidative stress in plasma and erythrocyte. Methods The rats were randomly divided into two groups,CUMS group and the control. The CUMS group received 28 days stress. Then 17 kinds of elements in plasma and erythrocyte were detected and the correlation ships between elements were analysed, then make comparison between two groups. Results The concentrations of Mo and B in plasma and Se in erythrocyte were higher in CUMS group than control（ P〈0. 05）. The levels of Sn,Cr,Th and Tl in plasma as well as Mn and Pb in erythrocyte were lower in CUMS group（ P〈0. 05）. The strength and direction of correlation ships between elements were changed in CUMS group. There had a negative correlation with Fe in plasma in control（ r =- 0. 581,P = 0. 018）,but a positive correlation in CUMS（ r = 0. 473,P = 0. 035）,the same with Th-Mg（ control： r =- 0. 610,P = 0. 012; CUMS： r = 0. 596,P = 0. 006）. Conclusion Concentrations and distribution of elements related to oxidative stress are changed by CUMS,most of theelements increased are related to anti-oxidative stress,while the reduced elements are prooxidative stress.

关 键 词：慢性轻度不可预见性应激 血浆 红细胞 化学元素 氧化应激 

分 类 号：R446.112[医药卫生—诊断学] Q58[医药卫生—临床医学]