鲍曼不动杆菌耐药性及bla_(OXA-23)基因研究  

Study on the drug resistance of Acinetobacter baumannii and bla_(OXA-23) gene

作　　者：肖美芳[1] 王昌富[1] 周义正[1] 邱小燕[1] 

机构地区：[1]华中科技大学附属荆州医院,湖北荆州434020

出　　处：《中国卫生检验杂志》2015年第17期2991-2993,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的研究耐碳青霉烯鲍曼不动杆菌blaOXA-23基因的分布情况,并探讨blaOXA-23基因与鲍曼不动杆菌对碳青霉烯类抗生素产生耐药性的相关性。方法按照全国临床检验规程,收集培养了178株亚胺培南耐药的鲍曼不动杆菌和4株敏感菌株,采用纸片扩散法(K-B法)检测鲍曼不动杆菌对16种临床常用抗菌药物的药敏试验,并利用聚合酶链式反应(PCR)对耐药基因blaOXA-23进行扩增,产物经DNA琼脂糖电泳后分离,并纯化测序。结果 PCR产物的DNA电泳显示扩增条带的大小与耐药blaOXA-23基因条带大小相吻合,测序结果与blaOXA-23基因序列比对,符合率为100%,携带blaOXA-23耐药基因的阳性菌株175株,阳性率为98.3%,且4株敏感(质控)菌株均未检出blaOXA-23耐药基因,且4株敏感(质控)菌株均未检出bla OXA-23耐药基因。结论本地区鲍曼不动杆菌对临床常用抗菌药存在严重耐药现象,本地区鲍曼不动杆菌对碳青霉烯类抗菌药物的高度耐药性与blaOXA-23基因的表达有关。Objective To investigate the distribution of carbapenem- resistant Acinetobacter baumannii blaOXA- 23 gene and to explore the correlation between blaOXA- 23 gene and Acinetobacter baumannii carbapenem antibiotic resistance. Methods A total of 178 imipenem- resistant Acinetobacter baumannii and 4 sensitive strains were collected and cultivated according to the national clinical laboratory procedures; antimicrobial susceptibility testing to 16 commonly used clinical Acinetobacter baumannii was carried out by means of Kirby- Bauer（ K- B） method; drug- resistant gene blaOXA- 23 was amplified by the polymerase chain reaction（ PCR）,and the DNA products were separated by agarose gel electrophoresis and sequenced after purification. Results The size of amplified DNA was close to the size do resistance blaOXA- 23 gene judged by the DNA electrophoresis; the sequence of amplified DNA was 100% identical to the resistance blaOXA- 23 gene accroding to the blast result; 98. 3% of multidrug- resistant Acinetobacter strains was found to be blaOXA- 23 gene positive and 4 sensitive（ QC） strains were blaOXA- 23 gene negative. Conclusion The drug resistance of Acinetobacter baumannii in this area to the common used antimicrobial is very serious,and the highly resistance of Acinetobacter baumannii to carbapenem antimicrobial drugs is related to blaOXA- 23 gene.

关 键 词：鲍曼不动杆菌 多重耐药 聚合酶链反应 blaOXA-23基因 

分 类 号：R446.5[医药卫生—诊断学]