Irisin通过激活AMPK促进小鼠骨骼肌细胞脂肪酸氧化  

Irisin increases fatty acid oxidation in mouse myocytes by activating AMPK

作　　者：辛超[1] 刘毅[1] 朱迪[1] 许倡涛 陶凌[1] 

机构地区：[1]第四军医大学西京医院心血管内科,陕西西安710032

出　　处：《心脏杂志》2015年第5期520-523,共4页Chinese Heart Journal

基　　金：国家杰出青年科学基金项目资助(81225001);国家重点基础研究发展计划项目资助(2013CB531204);新世纪优秀人才支持计划项目资助(NCET-11-0870)

摘　　要：目的研究irisin对C2C12小鼠骨骼肌细胞脂肪酸氧化的影响及机制。方法将培养好的C2C12细胞分为6组:对照组,irisin(20 ng/ml)组,irisin(200 ng/ml)组,irisin(2 000 ng/ml)组,irisin(2 000 ng/ml)+compound C(irisn孵育前30 min,20μmol/L)组,AICAR(AMPK激动剂,10μmol/L)组。使用3H标记的油酸孵育C2C12细胞,通过检测代谢产物放射性活度反映脂肪酸氧化水平;Western blot检测C2C12细胞与骨骼肌中AMPK和ACC-β的磷酸化水平。结果 1与对照组相比,irisin组C2C12细胞脂肪酸氧化水平升高(P<0.05)。2与对照组相比,irisin组AMPK和ACC-β的磷酸化水平增高(P<0.05或P<0.01)。3与Irisin组相比,irisin+compound-C组C2C12细胞脂肪酸氧化水平以及AMPK和ACC-β的磷酸化水平明显降低(P<0.05或P<0.01)。结论 Irisin通过激活AMPK促进小鼠骨骼肌细胞脂肪酸氧化。AIM To investigate the effect of irisin on fatty acid oxidation in myocytes and its underlying mechanism. METHODS C2C12 myocytes were divided into six groups randomly as follows： vehicle, irisin （20 ng/ml）, irisin （200 ng/ml）, irisin （2 000 ng/ml） irisin （2 000 ng/ml） ＋ compound C （30 min before irisin, 20 μmol/L） , AICAR （an AMPK activator, 10 μmol/L）. The phosphorylated and total AMPK and ACC were determined by Western blot and the oxidation of oleate was assayed by analyzing the oxidation products in the media after the cells were labeled with radioactive oleate. RESULTS 1 ） Compared with the control group, irisin significantly increased fatty acid oxidation （P 〈 0.05 ） in C2C12 myocytes. 2） Compared with control group, irisin significantly increased phosphorylated AMPK and ACC-[3 in C2C12 myocytes （P 〈 0. 05 or P 〈 0. 01 ）. 3） Compound-C （an AMPK inhibitor） inhibited the increase of fatty acid oxidation and phosphorylation of AMPK and ACC-β induced by irisin in C2C12 myocytes （P 〈 0. 05 or P 〈 0. 01 ）. CONCLUSION Irisin increases fatty acid oxidation in myocytes and skeletal muscle by activating AMPK.

关 键 词：irisin 脂肪酸氧化 胰岛素抵抗 骨骼肌细胞 

分 类 号：R543.1[医药卫生—心血管疾病]