Folding Kinetics of HDV Ribozyme with C13A:G82U and A16U:U79A Mutations  

作　　者：ZOU Yanjuan WANG Yujie GONG Sha ZHANG Wenbing 

机构地区：[1]School of Physics and Technology, Wuhan University

出　　处：《Wuhan University Journal of Natural Sciences》2015年第5期421-429,共9页武汉大学学报（自然科学英文版）

基　　金：Supported by the National Natural Science Foundation of China(31270761)

摘　　要：Gene mutations influence the folding kinetics of hepatitis delta virus （HDV） ribozyme. In this work, we study the effect of the double mutation on the folding kinetics of HDV ribozyme. By using the master equation method combined with RNA folding free energy landscape, we predict the folding kinetics of C13A：G82U and A16U：U79A mutated HDV sequences. Their folding pathways are identified by recursively searching the states with high net flux-in（out） population starting from the native state. The results indicate that the folding kinetics of C 13A：G82U mutation sequence is bi-phasic, which is similar to the wild type （wtHDV） sequence. While the folding kinetics of A16U：U79A mutation sequence is mono-phasic, it quickly folds to the native state in 30 s. Thus, the folding kinetics of double mutated HDV ribozyme depends on the mutation sites.Gene mutations influence the folding kinetics of hepatitis delta virus （HDV） ribozyme. In this work, we study the effect of the double mutation on the folding kinetics of HDV ribozyme. By using the master equation method combined with RNA folding free energy landscape, we predict the folding kinetics of C13A：G82U and A16U：U79A mutated HDV sequences. Their folding pathways are identified by recursively searching the states with high net flux-in（out） population starting from the native state. The results indicate that the folding kinetics of C 13A：G82U mutation sequence is bi-phasic, which is similar to the wild type （wtHDV） sequence. While the folding kinetics of A16U：U79A mutation sequence is mono-phasic, it quickly folds to the native state in 30 s. Thus, the folding kinetics of double mutated HDV ribozyme depends on the mutation sites.

关 键 词：hepatitis delta virus （HDV） ribozyme master equation method folding kinetics recursive search 

分 类 号：Q512.2[生物学—生物化学] Q754