小麦旗叶形态相关性状的QTL定位  

QTL Mapping for Morphological Traits of Flag Leaf in Wheat

作　　者：闫雪[1] 史雨刚[1] 梁增浩[1] 杨斌[1] 李晓宇[1] 王曙光[1] 孙黛珍[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《核农学报》2015年第7期1253-1259,共7页Journal of Nuclear Agricultural Sciences

基　　金：山西省自然基金(2013011034-2;2014011004-3);国家转基因生物新品种培育科技重大专项(2014ZX08002003-003);山西农业大学创新基金项目(2014YZ2-6)

摘　　要：为了发掘控制小麦旗叶形态性状的QTL,本试验以旱选10号/鲁麦14构建的包含有150个株系的DH群体为材料,采用完全随机区组设计,通过基于混合线性模型复合区间作图法,在干旱胁迫和正常灌溉2种水分条件下对小麦开花期旗叶长、叶宽、叶面积进行QTL定位。结果表明,共检测到3个旗叶长、4个旗叶宽和5个旗叶面积QTL,贡献率为6.06%～14.19%。其中q FLW-7A-1和q FLA-7B-1在2种水分条件下均被检测到,这2个QTL的表达不依赖于水分条件;其余的10个QTL只在1种水分条件下检测到,表明其表达依赖于水分条件。在5D染色体的标记区间Xgdm43～Xgwm174上发现有同时存在控制旗叶长和叶面积的QTL,在7B染色体的标记区间Xgdm68.1～WMC269.1上检测到同时控制旗叶长、叶宽和叶面积的QTL。本研究旨在检测不同环境条件下能够稳定表达的小麦旗叶形态性状QTL,为进一步精细定位和图位克隆提供理论参考。In order to detect QTLs for morphological traits of flag leaf in wheat, a population of 150 DH lines derived from Hanxuanl0 x Lumail4 were used in an experimental study of randomized complete block design with three replications. The objective of this study was to make a QTL analysis for flag leaf length, flag leaf width and flag area at flowering time in wheat under drought-stress and well-watered conditions using mixed composite interval mapping. The results showed that three additive effect QTLs for flag leaf length, four QTLs for flag leaf width and five QTLs for flag leaf area were identified. These QTLs can explain 6.06% -14. 19% of the phenotypic variances. Among them, qFLW- 7A - 1 and qFLA - 7B - 1 were detected under two water regimes, indicating that the expression of these two QTLs is not dependent on water conditions, while other ten QTLs identified in only one water condition showed that their expression may rely on moisture condition. The QTLs for flag leaf length and area were located in flanking marker Xgdm43 - Xgwm174 on chromosome 5D, and QTLs detected in flanking marker Xgdm68.1 - WMC269.1 on chromosome 7B controlled the flag leaf length, width and area together. The present study aimed at detecting QTLs controlling morphological traits of flag leaf in wheat under different environmental conditions to provide the theoretical reference for further fine mapping and map-based cloning.

关 键 词：小麦 DH群体 旗叶 形态性状 QTL 

分 类 号：S512.1[农业科学—作物学]