利用^(45)Ca探究BnTR1基因对油菜Ca^(2+)吸收的影响  

The Influences of Bn TR1 on Ca^(2+) Absorption of Rapes:Preliminary Investigation by ^(45)Ca

作　　者：宋波[1] 杨远友[1] 廖家莉[1] 杨吉军[1] 唐军[1] 刘宁[1] 

机构地区：[1]四川大学原子核科学技术研究所,四川成都610064

出　　处：《核农学报》2015年第7期1366-1373,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(10975103;21371124;91126013);国家自然科学基金国家基础科学人才培养基金(J1210004)

摘　　要：为探索钙离子与油菜胁迫响应机制的关系,利用45Ca放射性示踪剂研究基因Bn TR1(Brassica napus Thermal Resistant gene 1)对油菜钙吸收的影响。试验分析了从Bn TR1转基因油菜和野生型油菜中提取出来的原生质体对钙离子的吸收,比较其差异。结果表明,当胞外钙离子浓度低于0.9mmol·L-1时,转基因型原生质体比野生型原生质体吸收更多的钙离子;当外界钙离子浓度高于1mmol·L-1时,转基因型原生质体却比野生型原生质体吸收的钙离子更少;当胞外钙离子浓度大于30mmol·L-1时,转基因型原生质体吸收的钙离子大大低于野生型原生质体的吸收量;当加入钙离子通道阻塞剂氯化镧且其浓度从5μmol·L-1上升到20μmol·L-1时,转基因型原生质体会出现钙离子吸收峰值。综上,Bn TR1基因通过对细胞膜上钙离子通道更为灵敏和有效的调控,可以提高油菜对钙离子胁迫的耐受性。研究结果对研究Bn TR1基因的作用机制,以及其对油菜耐胁迫能力的影响提供了新的方向。To understand the relationship between Ca2＋ and stress response mechanism of rapes, 45Ca was used as a radioactive tracer to first explore the influences of Brassica napus Thermal Resistant gene 1 （ BnTR1 ） on Ca2＋ absorption of rapes. The study thoroughly analyzed the Ca2＋ absorption of protoplasts separated from the BnTR1 transgenic rapes and the non-transgenic rapes, and identified the difference between them. The study found that the protoplasts of the transgenic lines can absorb more Ca2＋ than the non-transgenic type if extracellular Ca2＋ concentration was less than 0.9 mmol.L-1 However, the reverse phenomenon was observed as the surrounding Ca2＋ concentration was more than 1 mmol·L-1. What＇s more, much less Ca2＋ was absorbed by the transgenic rape protoplasts than the non-transgenic ones, when the extracellular Ca2＋ concentration was greater than 30 mmol·L-1. It＇ s worth noting that, the peak value of Ca2＋ absorption of transgenic protoplasts occurred, when LaC13 increased from 5 to 20μmol·L-1. BnTR1 could improve plants＇resistance to Ca2＋ stress, through more sensitive and powerful control of calcium ions channel in cell membranes. This finding indicated a new direction about the future research on BnTR1 gene mechanism as well as its impact on the ability of resistance to stress of rapes.

关 键 词：BN TR1 45Ca 同位素示踪 原生质体 油菜 

分 类 号：S565.4[农业科学—作物学]