万寿菊不同品种的耐盐性综合评价  

Evaluation for Salt Tolerance of Marigold Cultivars

作　　者：段九菊[1] 张超[1] 郑梅梅 曹冬梅[1] 康黎芳[1] 王云山[1] 

机构地区：[1]山西省农业科学院园艺研究所,山西太原030031 [2]山西梅枝园艺公司,山西太原030025

出　　处：《核农学报》2015年第7期1406-1418,共13页Journal of Nuclear Agricultural Sciences

基　　金：山西省科技攻关项目(20110311017-1,20130311019-3)

摘　　要：为了解万寿菊(Tagetes erecta L.)常用品种的耐盐性,筛选耐盐品种,确定万寿菊耐盐评价和高效筛选的主要指标,本试验利用相关性分析法、主成分分析法、聚类分析法,对8个万寿菊品种的生长及形态指标、叶绿素、脯氨酸(Pro)和丙二醛(MDA)含量、电解质渗透率、荧光参数等生理指标的耐盐性进行综合评价。结果表明,盐胁迫下,万寿菊叶片的叶绿素及类胡萝卜素含量升高,Pro含量增加,最大光化学效率(Fv/Fm)和非光化学淬灭系数(NPQ)下降,MDA含量和电解质渗透率增加,植株生长受到抑制。与耐盐性弱的品种相比,耐盐性强的品种在盐胁迫下Pro积累量大,NPQ降低幅度小。相关性分析和主成分分析表明,复叶长、叶绿素b、NPQ、MDA是万寿菊耐盐性综合评价的主要指标。通过聚类分析将8个品种按照耐盐性分为4类,其中超美、大英雄耐盐性弱,印卡耐盐性中等,安提瓜、奇迹、梦之月、发现耐盐性较强,丰富耐盐性强。研究结果可为万寿菊耐盐种质创新及耐盐机制等方面提供理论参考。An experiment was conducted to explore the salt tolerance of marigold cuhivars which are widely grown in China, to select salt-tolerance cuhivar and to determine the main characters of salt tolerance evaluation and high effective screening. By the method of correlation analysis, principal component analysis and cluster analysis, a comprehensive evaluation was carried out on the salt-tolerance of plant growth and morphology indexes, physiological indexes such as leaf chlorophyll （Chl） content, proline （Pro） and malondialdehyde （MDA） content, electrolytic leakage （EC） and fluorescence parameters of eight marigold cultivars. Under salt stress, the content of Chl, carotenoid and Pro increased, maximum quantum efficiency （Fv/F~） and non-photo-chemical quenching （NPQ） decreased, the contents of MDA and EC increased together with reduced plant growth. In comparison to salt-sensitive cuhivar, salt-tolerance cuhivar showed a marked increase in Pro content but less decrease in NPQ. Correlation and principal component analysis suggest that the compound leaves length, Chl b, NPQ and MDA may be the most effective indexes for evaluation of salt tolerance of marigold cuhivars. These cultivars could be clustered into 4 groups, and the cuhivars Chaomeiand Great hero were sensitive to the salt stress, the cuhivar Inca belonged to moderately salt-tolerance group, the cuhivars Antigua, Qiji, Moonstruck, Discovery belonged to the strong salt-tolerance group, and the cultivar Fengfu belonged to the strongest salt- tolerance group. These results were useful for the studies of salt-tolerance germplasm innovation and physiological mechanism on marigold eultivars.

关 键 词：万寿菊 耐盐性 生长指标 生理指标 

分 类 号：S681.9[农业科学—观赏园艺]