尖齿臭茉莉HPLC指纹图谱研究  

HPLC Fingerprints of Clerodendrum lindleyi

作　　者：陈金梅 梅全喜[2] 彭丽华 王慧玲 成金乐 

机构地区：[1]中山市中智药业集团有限公司,广东中山528437 [2]广州中医药大学附属中山医院,广东中山528400

出　　处：《中药材》2015年第6期1183-1185,共3页Journal of Chinese Medicinal Materials

基　　金：中山市产学研结合项目(2011CXY004);广东省中医药局资助项目(20131078);中山市科技局重点资助项目(20132A002)

摘　　要：目的:建立尖齿臭茉莉的HPLC指纹图谱,为尖齿臭茉莉质量评价提供依据。方法:采用Agilent Zorbax C18(250 mm×4.6 mm,5μm)色谱柱;以乙腈-0.1%磷酸为流动相梯度洗脱;柱温为35℃;流速为1.0 m L/min;检测波长为327 nm。结果:建立了尖齿臭茉莉的HPLC指纹图谱共有模式,21个峰为11批样品所共有,确定为特征指纹峰。结论:该法精密度高,重复性和稳定性好,可为尖齿臭茉莉的质量评价提供可靠依据。Objective ：To establish the HPLC fingerprint of Clerodendrum lindleyi in order to provide the basis for its quality stand- ard. Methods：The chromatographic fingerprint was obtained with Angilent Zorbax C 18 （250 mm×4. 6 mm ,5 μm） column and gradiently eluted with acetonitrile-0. 1% phosphoric acid solution. The column temperature was maintained at 35 ℃. The flow rate was 1.0 mE/ min and the detection wavelength was 327 nm. Results： HPLC fingerprint of Clerodendrum lindleyi was established and 21 common peaks from 11 batches of samples were found. Conclusion：The method has good precision, stability and repeatability, which can provide reliable basis for quality evaluation of Clerodendrum lindleyi.

关 键 词：尖齿臭茉莉 HPLC指纹图谱 

分 类 号：R282.5[医药卫生—中药学]