益气除痰方对肺癌A549移植瘤细胞凋亡途径Caspase-4及DNA-PK的影响  

Effect of Yiqi Chutan Recipe on Caspase-4 and DNA-PK of Cell Apoptosis Approach in Transplanted Lung Cancer A549 Cells in Nude Mice

作　　者：李舒[1] 王淑美[1] 杨洋博君[1] 刘启欧 

机构地区：[1]重庆医科大学,重庆400016

出　　处：《中药材》2015年第6期1247-1250,共4页Journal of Chinese Medicinal Materials

基　　金：重庆市科委课题(渝科发计字[2013]22号)

摘　　要：目的:探讨益气除痰方是否通过内质网UPR介导的细胞凋亡途径发挥抗肿瘤作用。方法:建立40只肺癌A549裸鼠皮下移植瘤模型,于接种第7天将小鼠随机分为模型组(生理盐水组)、顺铂注射液组(0.002 g/kg)、益气除痰方(3.0 g/kg)+顺铂注射液(0.002 g/kg)组及益气除痰方低、高剂量组(3.0、6.0 g/kg),每组8只。中药灌胃给药,1次/d,连用21 d。顺铂注射液腹腔注射,1次/d,连用7 d。干预第22天,处死所有小鼠,测量瘤体质量、体积,采用免疫组化及Western blot法检测肿瘤组织中Caspase-4、DNA-PK的表达。结果:与模型组比较,益气除痰方各剂量组及益气除痰方+顺铂注射液组肿瘤体积、肿瘤质量显著降低,肿瘤组织中Caspase-4、DNA-PK表达均显著升高(P<0.01);益气除痰方+顺铂注射液组效果优于顺铂注射液组(P<0.05)。结论:益气除痰方对小鼠A549肺癌有一定抑制作用,其机制可能与通过升高Caspase-4、DNA-PK蛋白的表达介导细胞凋亡途径有关。Objective：To study the inhibitory effect of Yiqi Chutan Recipe on the transplanted tumor through endoplasmic reticu- lum UPR-mediated approach. Methods ：40 lung cancer A549 cells models transplanted in nude mice were established. On the 7th day of inoculation, mice were randomly divided into model group（ saline group）, Cisplatin group（0. 002 g/kg）, Yiqi Chutan Recipe low dose group（3.0 g/kg）, Yiqi Chutan Recipe high dose group（6.0 g/kg）and Yiqi Chutan Recipe（ 3.0 g/kg）with Cisplatin group（0. 002 g/ kg）. Each aforementioned group had eight mice. Mice were treated by Yiqi Chutan Recipe to gavage one time a day,for 21 days,and by Cisplatin Injection to intraperitoneal injection one time a day,for 7 days. On the 22th day, all mice were executed to death. Then each tumor＇s weight and volume were measured,and the expression of Caspase-4 and DNA-PK protein were detected through immunohisto- chemical method and Western blot method. Results：Compared with model group, the tumors＇ volume and weight of Yiqi Chutan Recipe high dose group and Yiqi Chutan Recipe with Cisplatin group were decreased, but the expressions of Caspase-4 and DNA-PK protein in tumors were increased（ P 〈 0. 01 ）. Yiqi Chutan Recipe with Cisplatin Group had the better effect（ P 〈 0.05 ）. Conclusion：Yiqi Chutan Recipe has a certain inhibitory effect on A549 lung cancer in mice and its possible mechanism is relevant to the increase of expression of Caspase-4 and DNA-PK protein.

关 键 词：益气除痰方 肺癌 细胞凋亡 Caspase-4 DNA-PK 

分 类 号：R285.5[医药卫生—中药学]