PB试验结合BBD响应面法优化浮萍多糖的提取工艺研究  

Optimization of Polysaccharide Extraction from Spirodela polyrrhiza by Plackett-Burman Design Combined with Box-Behnken Response Surface Methodology

作　　者：蒋征[1] 王红[1] 吴启南[1,2] 

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中药材》2015年第6期1283-1286,共4页Journal of Chinese Medicinal Materials

基　　金：江苏省大学生创新创业训练计划项目(20120315018);江苏高校优势学科建设工程资助项目(Noysxk 2010);江苏优秀科技创新团队"中药化学"(2011)

摘　　要：目的:优化浮萍多糖的提取工艺。方法:在单因素试验的基础上,采用Plackett-Burman试验法,对影响浮萍多糖提取率的5个因素进行筛选。根据PB试验结果,选取醇沉至终的乙醇体积分数、料液比、浸提温度为考察因素,运用Box-Behnken响应面法对浮萍多糖的提取工艺进行优化。结果:3个因素对浮萍多糖提取率影响的大小依次是:浸提温度>乙醇体积分数>料液比。最优工艺为:醇沉至终的乙醇体积分数为81%、料液比为1∶42、浸提温度为100℃、浸提时间为60 min、浸提4次。结论:运用Plackett-Burman法和Box-Behnken响应面法优选的浮萍多糖提取工艺稳定可行,可用于浮萍多糖的提取。Objective ：To optimize the processing of polysaccharide extraction from Spirodela polyrrhiza. Methods ： Five factors relat- ed to extraction rate of polysaccharide were optimized by the Plackett-Burman design. Based on this study, three factors, including alco- hol volume fraction, extraction temperature and ratio of material to liquid, were regarded as investigation factors by Box-Behnken re- sponse surface methodology. Results ：The effect order of three factors on the extraction rate of polysaccharide from Spirodela polyrrhiza were as follows ： extraction temperature, alcohol volume fraction, ratio of material to liquid. According to Box-Behnken response, the best extraction conditions were ： alcohol volume fraction of 81% , ratio of material to liquid of 1 ： 42, extraction temperature of 100℃, extrac- tion time of 60 min for four times. Conclusion ： Plackett-Burman design and Box-Behnkenresponse surface methodology used to optimize the extraction process for the polysaccharide in this study is effective and stable.

关 键 词：浮萍 多糖 Plackett-Burman试验法 Box-Behnken响应面法 

分 类 号：R284.1[医药卫生—中药学]