UPLC/Q-TOFMS法对何首乌配方颗粒化学成分的快速分析  

Rapid Identification of Chemical Components in Polygonum multiflorum Formula Granules by UPLC/Q-TOF MS

作　　者：胥爱丽 董玉娟[2] 陈昭 江洁怡 李素梅 李养学 

机构地区：[1]广东省中医药工程技术研究院/广东省中医药研究开发重点实验室,广东广州510095 [2]广州中医药大学,广东广州510405

出　　处：《中药材》2015年第6期1287-1289,共3页Journal of Chinese Medicinal Materials

基　　金：广东省中医药局科研课题(20132078);广东省教育部科技部产学研结合项目(2012B091100183);广东省科学技术厅项目(2012A030100017);佛山市科技创新专项资金(2013GQ100032)

摘　　要：目的:建立一种快速、简便、可靠的方法对何首乌配方颗粒的化学成分进行快速分析鉴定。方法:采用超高效液相色谱-四极杆-飞行时间高分辨质谱(UPLC/Q-TOF MS)联用技术,以Agilent Eclipse Plus C18RRHD(100mm×2.1 mm,1.8μm)为色谱柱;乙腈-水为流动相进行梯度洗脱;流速0.4 m L/min;柱温25℃;质谱定性采用飞行时间质谱,负离子模式扫描。结果:通过将各色谱峰的质谱数据与数据库匹配及对参考文献的数据分析,鉴定出何首乌配方颗粒中的5个化合物。结论:该方法准确、快速、灵敏,为何首乌配方颗粒的质量控制提供参考。Objective ：To establish a simple and reliable method for rapid separation and identification of chemical components in Polygonum multiflorum Formula Granules. Methods：An ultra-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometric method（ UPLC/Q-TOF MS）was used. The separation was performed on an Agilent Eclipse Plus C18 RRHD（ 100 mm × 2. 1 mm, 1.8 μm）column with a mobile phase of water and acetonitrile in a gradient elution mode. The flow rate was 0. 4 mL/min and the column temperature was maintained at 25 ℃. TOF MS was applied for qualitative analysis under positive ion mode. Results：Five com- pounds were identified by the time of flight mass spectrometry and literature data. Conclusion：This method is accurate, rapid and sensi- tive,it can provide reference for the quality control of Polygonum multiflorum Formula Granules.

关 键 词：超高效液相色谱-四级杆-飞行时间质谱 何首乌配方颗粒 化学成分 

分 类 号：R284.1[医药卫生—中药学]