玉米碳代谢关键酶活性的遗传分析  

Genetic Analysis of Carbon Metabolism Related Enzymes Activity in Maize

作　　者：杨双[1,2] 韩晓日[1] 

机构地区：[1]沈阳农业大学土地与环境学院,辽宁沈阳110161 [2]沈阳市农业科学院,辽宁沈阳110034

出　　处：《核农学报》2015年第8期1455-1463,共9页Journal of Nuclear Agricultural Sciences

基　　金：2014年沈阳市科技局公益类科技招标项目(E140025)

摘　　要：为研究玉米碳代谢关键酶PEPC和SPS活性的遗传特性,应用主基因+多基因混合遗传模型理论和4世代联合分离分析方法,对沈3336×沈3265组合4个世代的灌浆期穗位叶PEPC和SPS活性进行遗传分析。结果表明,PEPC和SPS活性的遗传模型分别为B-1模型(2对主基因的加性-显性-上位性模型)和E-1模型(2对加性-显性-上位性主基因+加性-显性多基因混合模型)。调控PEPC活性的主基因遗传率为36.5%,调控SPS活性的主基因遗传率为17.7%,多基因遗传率为35.9%。结果为玉米碳代谢相关性状的遗传改良提供理论依据,也为下一步开展QTL定位研究奠定基础。In order to study the genetic characteristic of the carbon metabolism related enzymes activity in maize,the genetic analysis of the PEPC and SPS activity in ear leaf at filling stage in maize were conducted by mixed major gene plus polygene genetic models and the joint segregation analysis method of four generations（ P1,P2,F1,F2∶ 3） derived from Shen3336 × Shen3265. The results showed that at filling stage the genetic models of PEPC and SPS activity in ear leaf were B- 1（ two major genes with additive-dominance-epistasis effects） and E- 1（ two major genes with additivedominance-epistasis effects and polygenes with additive-dominance effects）,respectively. The major genes heritability of PEPC activity was 36. 5%. The major genes heritability of SPS activity was 17. 7% and polygenes heritability was35. 9%. The results explained the genetic regularity of the carbon metabolism related enzymes activity in maize,and helped for carrying out next research of QTL mapping of these enzymes activity.

关 键 词：玉米 磷酸烯醇式丙酮酸羧化酶(PEPC) 蔗糖磷酸合成酶(SPS) 遗传分析 

分 类 号：S513[农业科学—作物学]