条锈菌侵染后不同抗性小麦叶片蛋白差异表达研究  

Analysis of Differentially Expressed Proteins in Leaf of Different Disease-resistance Wheat Varieties After Infected With Puccinia striiformis

作　　者：孟亚雄[1,2] 李佳春[1,2] 汪军成[1,2] 张海娟[1,2] 赖勇[1,2] 司二静[1,2] 杨柯[1,2] 马小乐[1,2] 李葆春[1,3] 王化俊[1,2] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《核农学报》2015年第9期1649-1656,共8页Journal of Nuclear Agricultural Sciences

基　　金：甘肃省自然基金(1208RJZA136);甘肃省干旱生境作物学重点实验室开放基金(GSCS201007)

摘　　要：为从蛋白组学水平揭示不同抗性小麦品种对条锈病的响应机制,以3个不同抗性小麦品种为材料,从蛋白质组调控的角度分析了条锈菌侵染后不同抗性小麦品种叶片蛋白质的表达差异。结果显示,侵染菌种CY32与混合菌后,高抗条锈病品系天9524、慢条锈病品种兰天15号和高感条锈病品种铭贤169叶片中一些蛋白的表达呈现出显著上调或下调,利用MALDI-TOF/TOF MS/MS质谱鉴定和MASCOT数据库检索,从3个小麦品种中共注释出16个差异表达蛋白点,其中铭贤169中7个,兰天15号中6个,天9524中3个。按照其功能分类,这些差异表达蛋白点分别参与了叶绿体光合作用,蛋白质合成,抗病响应等生理活动。这些差异表达蛋白可能与小麦响应条锈菌侵染过程有关,为抗条锈病相关基因的克隆与抗病响应机制的研究提供参考。In order to elucidate the proteomic basis of wheat varieties with different response to P. striiformis. The proteomic approach was used to analyze the changes of differentially expressed leaf proteins during different stages after infected with the stripe rust CY32 and mixed-Puccinia striiformis in three different resistance wheat varieties. Result showed that leaf proteins of Tian 9524（ immune variety）, Lantian 15（ resistant cultivar）, and Mingxian 169（susceptible variety） were changed significantly before and after inoculated with P. striiformis. A total of 16 differentially expressed protein detected with two-dimensional electrophoresis（2- DE） were identified by MALDI-TOF /TOF-MS / MS and protein database searching by MASCOT program,including three in Tian 9524,six in Lantian 15,and seven in Mingxian 169. The functions of these 16 proteins were related to photosynthesis,protein synthesis and response to resist disease,etc. These differentially expressed proteins maybe related to the response to infection by P. striiformis.This result provided the basic information to further reveal the resistant mechanisms and clone the resistant-related genes in wheat to Puccinia striiformis.

关 键 词：蛋白质组学 小麦 条锈菌 抗病机理 差异表达 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]