SPE-HPLC法测定水中硝基苯酚类化合物  

Determination of Nitrophenols in Water by Solid Phase Extraction and High Performance Liquid Chromatography

作　　者：秦承华[1] 王晶晶 张军 彭华 王玲玲 南淑清 李红亮 吴立业 

机构地区：[1]中国环境监测总站国家环境保护环境监测质量控制重点实验室,北京100012 [2]河南省环境监测中心,河南郑州450004

出　　处：《中国环境监测》2015年第4期86-90,共5页Environmental Monitoring in China

基　　金：国家863高技术研究发展计划(2013AA06A308)

摘　　要：建立了以固相萃取为前处理条件,用液相色谱法测定水中10种硝基苯酚类化合物的分析方法。实验使用HLB（6 L/150 g）固相萃取柱富集水样中的目标化合物,二氯甲烷与乙酸乙酯体积比1∶2的混合溶剂洗脱,采用Phenyl柱（4.6 mm×250 mm,5μm）分离目标化合物,以乙腈（1%甲酸）/水（1%甲酸）为流动相进行梯度洗脱,二极管阵列检测器检测。10种硝基苯酚类物质在0.02-10 mg/L范围内呈现良好的线性。方法检出限为0.1-0.3μg/L,水样加标相对标准偏差为5.19%-18.2%,平均加标回收率为49.8%-124%。该方法适用于水中10种硝基苯酚类化合物的测定。A method had been established to determine ten kinds of nitrophenols in water by solid phase extraction and high performance liquid chromatography. In this study, HLB SPE column（6 L/150 g）was used to extract the target compounds and the target compounds on the HLB SPE column were desorbed by a mixed solvent of dichloromethane and ethyl acetate（ 1：2, V： V）. Then,the target compounds were separated by using a Phcnyl column （5 μm,4.6 mm × 250 mm） with acetonitrile （ 1% formic acid） /water （1% formic acid） as the mobile phase and were detected by diode array detector. The study showed that decent linearity between concentration and peak area was achieved in the concentration range of 0. 02-10 mg/L for the ten target compounds. The method detection limit for each target compound ranged from 0.1 μg/L to 0. 3 μg/L. The recovery for the samples is between 49.8% -124% with the relative standard deviation of 5.19% -18.2%. This method was suitable to determine the ten kinds of nitrophenols in water.

关 键 词：固相萃取 硝基苯酚类化合物 高效液相色谱 

分 类 号：X830.2[环境科学与工程—环境工程]