山莨菪根HPLC指纹图谱研究  

HPLC Fingerprint of Anisodus tanguticus Root

作　　者：蒋运斌[1] 苟琰 袁茂华[1] 马逾英[1] 周娟 伍丕娥 

机构地区：[1]成都中医药大学药学院/中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 [2]四川省食品药品检验检测院,四川成都610079

出　　处：《中药材》2015年第5期957-961,共5页Journal of Chinese Medicinal Materials

基　　金：四川省科技支撑计划项目(2013SZ0115)

摘　　要：目的：建立山莨菪根的HPLC指纹图谱,为其药材质量控制提供一种可靠的方法。方法：采用UltimateAQC18（250mm×4.6mm,5μm）色谱柱;以乙腈-10mmol/LKH2PO4缓冲液（用H3PO4调节pH至3.0）为流动相梯度洗脱;流速1.0mL/min;柱温30℃;检测波长210nm;进样量10μL。并对测得指纹图谱进行相似度评价和主成分分析。结果：18批不同山莨菪根样品共确立了15个共有峰,建立了山莨菪根的HPLC对照指纹图谱,结合保留时间和紫外光谱,指认了樟柳碱、东莨菪碱、山莨菪碱、阿托品4个特征峰。18批野生山莨菪根的相似度在0.891~0.987之间。对15个共有峰进行主成分分析,综合评判得分在-0.85~0.89之间。结论：所建立的指纹图谱具有良好的精密度、重复性和稳定性,为山莨菪根的鉴别和质量控制提供了更全面的信息。Objective： To establish an HPLC fingerprint of Anisodus tanguticus root for its quality control. Methods： The analysis was carried out on a Ultimate AQ C18（ 250 mm × 4. 6 mm,5 μm） column with the gradient elution of acetonitrile and KH2PO4 buffer solution,whose p H was adjusted to 3. 0 with phosphoric acid. The flow rate,column temperature,detection wavelength and injection volume was 1. 0 m L / min,30 ℃,210 nm and 10 μL separately. The similarity evaluation and principal component analysis were used to analyze HPLC fingerprint of Anisodus tanguticus root. Results： HPLC fingerprint of Anisodus tanguticus root was established with 15 common peaks by determining 18 batches of Anisodus tanguticus root samples. Four characteristic peaks,anisodine,scopolamine,anisodamine and anisodamine,were confirmed by comparing their retention time and UV spectrum with standard reference substances. The similarities of 18 batches of Anisodus tanguticus root were between- 0. 891 and 0. 987. Comprehensive evaluation scores of 18 batches of Anisodus tanguticus root were between- 0. 85 and 0. 89 by principal component analysis. Conclusion： The established HPLC fingerprint has good precision,repeatability and stability,which can provide more comprehensive information for identification and quality control of Anisodus tanguticus root.

关 键 词：山莨菪根 HPLC指纹图谱 质量评价 

分 类 号：R282.5[医药卫生—中药学]