并发冠心病的血液透析患者血浆中精氨酸酶Ⅰ水平变化的研究  

Changes of plasma arginase I levels in hemodialysis patients with coronary heart disease

作　　者：温红梅[1] 杨磊[1] 任笑丽[1] 孙亦兵[1] 阎敏娜 

机构地区：[1]河北北方学院附属第一医院血液透析室,张家口075000 [2]河北北方学院附属第一医院检验科,张家口075000

出　　处：《临床肾脏病杂志》2015年第8期485-488,共4页Journal Of Clinical Nephrology

基　　金：张家口市科学技术研究与发展计划项目(NO.1421058D)

摘　　要：目的测定血液透析（hemodialysis，HD）患者血浆中精氨酸酶Ⅰ（arginase Ⅰ，ArgⅠ）水平，确定其是否与HD患者合并冠心病（coronary heart disease，CHD）情况有关。方法选择2011年3月至2014年4月间于河北北方学院附属第一医院进行维持性血液透析治疗的45例HD患者和45例健康对照者作为研究对象，并根据CHD合并情况将HD患者进一步分为合并CHD组和不合并CHD组。用ELISA试剂盒测定所有研究对象血浆中Arg Ⅰ和两种炎性细胞因子[白细胞介素6（interleukin6，IL-6）和肿瘤坏死因子α（tumor necrosis factord，TNF-α）]水平。结果HD患者与对照组血浆中Arg工没有显著的差异[（12．97±7．32）ng／ml比（9．63±4．65）ng／ml，P〉0．05]；HD合并CHD患者ArgⅠ水平较不合并CHD组显著升高[（19．72±10．54）ng／ml比（10．01±7．73）ng／ml，P〈0．05]，较对照组也显著增高[（19．72±10．54）ng／ml比（9．63±4．65）n4g／ml，P〈0．05]。HD患者血浆中IL-6和TNF-α水平也显著高于对照组[IL-6：（28．66±10．13）pg／ml比（4．17±2．42）．pg／ml，P〈0．05；TNF-α：（51．39±6．53）pg／ml比（15．22±2．1）pg／ml，P〈0．05]；HD合并CHD组和不合并CHD组患者血浆IL-6含量较对照组显著增高[分别为（25．62±7．27）pg／ml、（29．45±11．36）pg／ml和（4．17±2．42）pg／ml]，合并CHD与否对HD患者血浆Il-6水平影响不大（t=0．441，P〉0．05）；FIB合并CHD组和不合并CHD组患者血浆TNF-α含量较对照组显著增高[分别为（33．57±6．72）pg／ml、（37．69±7．57）pg／ml和（15．22±2．1）pg／ml]，合并CHD与否对HD患者血浆TNF-α的水平无影响（t=1．265，P〉0．05）。ArgⅠ与IL-6水平无显著相关性（r=-0．136，P〉0．05），与TNF-α水平也无显著相关性（r=-0．135，P〉0．05）；且IL-6和TNF-α间也没有显著相关性（r=0．069，P〉0．05）。结论HD患者血浆中ArgⅠ的水Objective To determine the correlation between the arginase Ⅰ （Arg Ⅰ ） level and coronary heart disease （CHD） in hemodialysis （HD） patients. Methods Forty-five HD patients and 45 healthy volunteers were chosen from May 2011 to Apr. 2014 in the First Affiliated Hospital of Hebei North University. HD patients were sub-divided into CHD subgroup and non-CHD sub-group. Plasma Arg Ⅰ , IL-6 and TNF-α were measured by ELISA kits. Results There was no statistically significant difference in gender and age between HD patients and healthy controls （P〉0. 05）. There was no obvious difference in Arg Ⅰ level between HD patients and healthy controls （12. 97 ± 7. 32 vs. 9. 63 ±4. 65 ng/ml, P〉0. 05）. Arg Ⅰ level in HD patients with CHD was higher than HD patients without CHD （19. 72 ± 10. 54 vs. 10. 01 ±7. 73 ng/ml, P〈0. 05）, and higher than healthy controls （9. 63± 4. 65 ng/ml, P〈0. 05）. IL-6 and TNF-α levels were increased in HD patients as compared with healthy controls （IL-6： 28. 66 ± 10. 13 vs. 4. 17 ±2. 42 pg/ml, P〈0. 05∴TNF-α： 51.39 ± 6. 53 vs. 15.22 ± 2. 1 pg/ml, P〈0. 05）, but showed no significant difference between HD patients with or without CHD （IL-6.. 25.62 ± 7. 27 vs. 29. 45 ± 11.36 pg/ml; TNF-α： 33. 57 ± 6. 72 vs. 37. 69 ± 7. 57pg/ml）. IL-6 and TNF-α levels had no significant correlation with Arg Ⅰ level in HD patients. Conclusions Arg Ⅰ levels may be associated with the occurrence of CHD in HD patients.

关 键 词：血液透析 冠心病 精氨酸酶 白细胞介素6 肿瘤坏死因子Α 

分 类 号：R459.5[医药卫生—治疗学]