流式细胞仪检测线粒体膜电位方法的研究  

Study on methods for detecting mitochondrial membrane potential by flow cytometry

作　　者：岳磊[1] 张垚[1] 张楠曦[1] 

机构地区：[1]哈尔滨工业大学生命科学与技术学院,哈尔滨150080

出　　处：《哈尔滨商业大学学报（自然科学版）》2015年第4期393-397,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：通过流式细胞仪进行检测,研究选择每种检测线粒体膜电位(Δψm)荧光染料的适用条件.选用罗丹明123、Di OC6(3)、TMRE、JC-14种荧光染料,通过对紫外线诱导后细胞的线粒体膜电位进行检测,分析每种染料的检测条件.结果表明,罗丹明123检测到的Δψm变化较大,这可能与罗丹明123适用于检测细胞急性损伤中线粒体膜电位变化相关.通过比较实验方法,为将来采用流式细胞仪检测线粒体膜电位提供更准确的实验方法.This paper aimed to determine the applicable conditions for detecting mitochondri -al membrane potential （Δψm ） of fluorescent dyes by flow cytometry .Rhodamine 123, Di-OC6 （3） , TMRE, JC-1 were chose and used to analyze the conditions of each dye for the mitochondria membrane potential detection through the induction of UV .The results showed that the change of Δψm detected by Rhodamine 123 was larger than other dyes , which may be related to the adaption of Rhodamine 123 to acute cell damage .By comparing the experi-mental method , it is more accurate for the future of the mitochondrial membrane potential de-tection by flow cytometry .

关 键 词：流式细胞仪 线粒体膜电位 罗丹明123 DiOC6(3) TMRE JC-1 

分 类 号：R446[医药卫生—诊断学]