Caco-2细胞单层模型的建立及其评估  

Establishment of Caco-2 cell monolayer model

作　　者：孙旭[1,2] 罗余萍[2] 

机构地区：[1]中国食品药品检定研究院国家药物安全评价监测中心药物非临床安全评价研究北京市重点实验室,北京100176 [2]南昌大学医学院临床药理研究所,江西南昌330006

出　　处：《药品评价》2015年第14期33-35,39,共4页Drug Evaluation

摘　　要：目的:建立完整的Caco-2细胞单层模型,以利于药物转运的研究。方法:通过测量细胞生长过程中跨膜电阻(TEER)值和碱性磷酸酶活性,评估Caco-2细胞单层模型是否建立成功。结果:细胞接种到Transwell板后的第7天,TEER值就已达到209.75Ω·cm2,第10天后,几乎成线性上升,直到第22天到达一个稳定值627Ω·cm2;细胞生长的第3天,磷酸酶活性非常低,第15天时磷酸酶活性比较高。到了第22天时,磷酸酶活性进一步增加,AP/BL为第15天时的2倍。结论:建立的Caco-2细胞单层模型致密且完整,检测细胞生长过程中TEER值和碱性磷酸酶活性,可用于评估Caco-2细胞单层模型建立成功。Objective： Building a complete Caco-2 cell monolayer model. Methods： Estimated the success of Caco -2 cell monolayer model by measured the TEER value and activity of alkaline phosphatase. Results： Day 7 after Caco- 2 cell planted on transwell plate, the TEER value was 209.75 Ω·cm^2. It increased almost linearly after day 10, and attained a stable value of 627 Ω·cm^2 in day 22. The activity of alkaline phosphatase was very low in day 3, but it becames much higher in day 15. AP/BL in day 22 was 2 times of day 15. Conelusiom The Caco-2 cell monolayer model was dense and complete, and can be used to transport experiment.

关 键 词：CACO-2细胞单层模型 跨膜电阻(TEER)值 碱性磷酸酶 

分 类 号：R965[医药卫生—药理学]