猪传染性胃肠炎病毒N蛋白在猪小肠黏膜上皮细胞中的表达及对细胞周期的影响  

Expression of Protein N of Porcine Transmissble Gastroenteritis in Swine Intestinal Epithelial Cells and Effect and Influence on Cell Cycle

作　　者：常嵘[1] 张琪[1] 何亚鹏[1] 童德文[1] 许信刚[1] 

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2015年第8期7-10,共4页Progress In Veterinary Medicine

基　　金：西北农林科技大学"基本科研业务费专项资金项目"(QN2012017)

摘　　要：为鉴定猪传染性胃肠炎病毒(TGEV)N蛋白在猪小肠黏膜上皮细胞(SIEC)中的表达并检测其对细胞周期的影响,将构建的含有TGEV N基因的重组质粒pEGFP-N1-N采用脂质体介导法瞬时转染SIEC,通过RT-PCR和Western blot方法分别从分子水平和蛋白水平检测N蛋白的表达,采用流式细胞术检测N蛋白对IEC细胞周期的影响。结果表明,N蛋白在SIEC中成功表达,并使细胞的S期延长。研究结果为深入研究TGEV N蛋白功能提供新的理论依据,并为进一步探讨TGEV的致病机理奠定基础。To identify the expression of the N gene of TGEV in porcine swine intestinal epithelial cells(SIEC)and the effect of N gene on cell cycle,recombinant plasmids pEGFP-N1-N were transfected into SIEC using lipofectamine 2000.RT-PCR and Western blot were used to detect the expression of N gene through the level of molecule and protein respectively.The effect of N protein on cell cycle progression was investigated by flow cytometry assay.The results showed that the N gene of TGEV was successfully expressed in SIEC,resulting in the prolong of S-phase cell cycle.This research may contribute to establish the theoretical basis for further studying the function and the pathogenesis mechanism of N protein of TGEV.

关 键 词：猪传染性胃肠炎病毒 N蛋白 猪小肠黏膜上皮细胞 细胞周期 

分 类 号：S852.659.6[农业科学—基础兽医学] S852.21[农业科学—兽医学]