黄花棘豆2种生物碱成分的鉴定  

Alkaloids from Poisonous Plant Oxytropisochrocephala

作　　者：谭承建[1] 刘丽娜[2] 胡毕斯哈勒图 汤洪敏[1] 赵宝玉[4] 

机构地区：[1]贵州民族大学化学与环境科学学院,贵州贵阳550025 [2]贵阳医学院生物与工程学院,贵州贵阳550025 [3]内蒙古阿拉善孪井滩动检站,内蒙古阿拉善750300 [4]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《动物医学进展》2015年第8期71-73,共3页Progress In Veterinary Medicine

基　　金：国家"十二五"公益行业(农业)科研专项(201203062);国家自然科学基金项目(31360084);国家留学基金项目;贵州省科技厅科学技术基金(黔科J字[2010]2273);贵州民族大学引进人才科研基金项目

摘　　要：为分析我国天然草地主要有毒植物黄花棘豆的生物碱成分,为其毒理学、药理学和资源综合利用提供理论依据,本试验采用硅胶柱层析、Sephadex LH-20、RP18、半制备HPLC技术和现代波谱技术对黄花棘豆生物碱成分进行研究。结果表明,从黄花棘豆正丁醇萃取部位分离得到2个极性较大的生物碱成分,通过现代波谱技术鉴定为(+)-9α-羟基苦参碱和(-)-9α-羟基槐果碱。2种化合物均为首次从棘豆属植物中分离得到。In order to make clear the alkaloid constituents of poisonous plant Oxytropis ochrocephala dis-tributed in natural grassland and provide the further theoretical basement for toxicology,pharmacology and comprehensive utilization of this plant,the BuOH exact of Oxytropis ochrocephala was purified by means of various chromatographic techniques such as silica gel column,Sephadex LH-20 gel,RP-18 and semipreparative HPLC.As the result,two alkaloids were obtained and their structures were elucidated by spectral analysis as (+)-9α-hydroxymatrine (1),(-)-9α-hydroxysophocarpine (2).Compounds 1-2 were i-solated from the plants genus Oxytropis for the first time.

关 键 词：黄花棘豆 生物碱 疯草 有毒植物 

分 类 号：S856.9[农业科学—临床兽医学]