硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂的研制  

Development of chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate

作　　者：黄明聪 张晓琍[2] 林光华[2] 翁祖星 孙旭东 葛胜祥[3] 张军[3] 

机构地区：[1]厦门万泰凯瑞生物技术有限公司,福建厦门361022 [2]福建医科大学附属协和医院检验科,福建福州350001 [3]厦门大学国家传染病诊断试剂与疫苗工程技术研究中心,福建厦门361102

出　　处：《分子诊断与治疗杂志》2015年第5期302-305,322,共5页Journal of Molecular Diagnostics and Therapy

基　　金：国家高技术研究发展计划(863计划)(2011AA02A101)

摘　　要：目的研制硫酸脱氢表雄酮化学发光免疫定量检测试剂。方法利用硫酸脱氢表雄酮人工完全抗原免疫小鼠,通过杂交瘤技术制备特异性抗硫酸脱氢表雄酮单克隆抗体,采用竞争抑制法建立硫酸脱氢表雄酮化学发光免疫定量检测试剂。结果筛选获得了27株稳定分泌抗硫酸脱氢表雄酮的单克隆抗体细胞株,建立了化学发光微粒子免疫法定量测定硫酸脱氢表雄酮的试剂盒雏形,与雅培公司的硫酸脱氢表雄酮定量检测试剂在检测临床标本上的相关系数r达0.99以上。结论本研究为国产化硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂盒的研发奠定了基础。Objective To develop chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate. Methods Mice were immunized by dehydroepiandrosterone sulfate artificial complete antigen.Monoclonal antibodies against dehydroepiandrosterone sulfate were prepared by hybridoma technique.Chemiluminescent microparticle immunoassay for quantitative determination of dehy- droepiandrosterone sulfate was developed based on competitive binding method. Results 27 monoclonal antibodies were obtained. Chemiluminescent microparticle immunoassay for quantitative determination of debydroepiandrosterone sulfate prototype kit was established. The coefficient r of testing specimens with reagent from Abbott company was over 0.99. Conclusion Development of domestic chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate reagent was laid foundation by this study.

关 键 词：硫酸脱氢表雄酮 化学发光微粒子免疫法 定量试剂盒 

分 类 号：R446.6[医药卫生—诊断学]