UF-100尿沉渣分析、干化学分析及尿液培养联合检测在尿路感染诊断中的意义  

Flow cytometry（Sysmex UF-100）, dipsticks and urine cultures in diagnosis of urinary tract infection

作　　者：谢珊珊[1] 

机构地区：[1]龙岩市第一医院检验科,福建龙岩364000

出　　处：《医学检验与临床》2015年第4期22-24,共3页Medical Laboratory Science and Clinics

摘　　要：目的：探讨UF-100尿沉渣分析、干化学分析和尿液培养在尿路感染诊断中的意义。方法：对某市某医院658份中段尿标本做细菌培养和沉渣分析、干化学分析,并比较结果。结果：尿细菌培养阳性235例，阳性率为35.7%；与尿细菌培养比较，UF-100及US-2100R不同组合对尿路感染的诊断价值如下：假阳性率为4.49%～13.10%；假阴性率为20.41%～24.01%；敏感性32.77%～44.00%；特异性79.63%～93.23%；阳性预测值为49.68%～71.21%；阴性预测值为68.59%～73.39%；准确率64.13%～73.05%。结论：在尿路感染的实验室检查中，UF-100和US-2100R结果阳性时,对尿路感染有一定的预见性,但不能肯定尿路感染，仍须进行细菌培养以进一步确认；结果阴性时，也无法完全排除尿路感染，也就是干化学及尿沉渣结果无法明确预见尿细菌培养结果，诊断尿路感染仍需尿细菌培养。Objective：To explore the clinic value about combined flow cytometry （Sysmex UF-100） and dipsticks and urine cultures in diagnosis of urinary tract infection. Methods：658 urine samples were collect from the patients who suspected urinary tract infection, samples were tested with dipsticks, the flow cytometry and cultures, then compare all results.Results：Urinary cultures identified 235 （35.7%） samples had a pure growth of more than 10（5）cfu/ml, compared with cultures, the two methods of flow cytometry （Sysmex UF-100） and dipsticks showed a result as follows：the false positive rate（FPR） was 4.49%~13.10%;the false negative rate（FNR） was 20.41%~24.01%; the sensitivity was 32.77%~44.00%; the specificity was 79.63%~93.23%; the positive predictive value was 49.68%~71.21%;the negative predictive value was 68.59%~73.39%;the accurate rate was 64.13%~73.05%. Conclusions：Flow cytometry （Sysmex UF-100） and dipsticks （Sysmex US-2100R） ’s positive rusults performed a certain predictability in urinary tract infection, but negative rusults detected by flow cytometry （Sysmex UF-100） and dipsticks （Sysmex US-2100R） also can ’t exclude urinary tract infection, whether urinary tract infections requiring the detection of urinary culture.

关 键 词：尿路感染 UF-100 干化学分析 细菌培养 

分 类 号：R446.12[医药卫生—诊断学]