新白膜法制备浓缩血小板的质量分析  

Quality analysis of new buffy coat method preparation platelet concentrate

作　　者：朱仕清[1] 危燕芬[1] 

机构地区：[1]广州血液中心花都区血站,广东广州510800

出　　处：《医学检验与临床》2015年第4期72-74,共3页Medical Laboratory Science and Clinics

摘　　要：目的：探讨浓缩血小板的最佳制备方法，同时，对影响浓缩血小板质量的因素进行分析，以提高浓缩血小板的质量，确保临床应用疗效。方法：随机抽取6h内采集的400ml全血200袋，分别采用富浆法（PRP）及新白膜法（BC）制备浓缩血小板，以PRP制备的浓缩血小板为对照组（A组），用新BC法第一次离心后制备的白膜层，分别放置在血小板震荡仪中保存2h（B组）、6h（C组）、12h（D组）、26h（E组）进行第二次离心，制备出浓缩血小板，作为实验组。检测各组的血小板计数（PLT）、白细胞及红细胞残余量、pH值及细菌培养等指标，观察不同制备方法及白膜层不同存放时间对浓缩血小板的质量影响。结果：采用新BC法（B组除外）制备的浓缩血小板较PRP法含量高，其中，以D组制备的浓缩血小板含量最高，C、E组制备的浓缩血小板含量高于A组，差异有统计学意义。各组浓缩血小板细菌培养均无细菌生长。结论：采用新BC法制备的浓缩血小板优于PRP法，且采用新BC法制备的浓缩血小板可以在传统的6-8h以外的时间进行，超过24h制备仍能达到质量要求。Objective：To improve the quality of the platelets concentrate and ensure the clinic therapy by exploring the best way to prepare platelets concentrate as well as analyzing the factors on affecting its quality. Methods：Select 50 bags of whole blood with 400 ml within 6h at random which are respectively used to prepare the platelets concentrate by PRP and new BC. The platelets concentrate prepared by PRP comes from the control as Group A. The Buffy coat prepared and centrifuged by new BC for the first time are respectively placed and kept in the Platelet concussion instrument for 2h as Group B, 6h as Group C, 12h as Group D, and 26h as Group E. After that, they are centrifuged for the second time to prepare the platelets concentrate which came from experimental group. Testing the PLT, count the Residual Volume of white blood cells and red blood cells, pH and bacterial culture, observing different preparation or observing the effect of different periods of keeping Buffy coat to platelets concentrate.Results：The amount of platelets concentrate by new BC（except Group B） is more than the PRP, including Group D with the most platelets concentrate, Group C and Group E more than Group A. The difference is statistically significant. No bacteria grow up on every bacterial culture. Conclusions：The platelets concentrate prepared by new BC is superior to the one by PRP. In addition, the platelets concentrate by new BC can be done over 6-8h, even can reach certain quality over 24h.

关 键 词：新白膜法 浓缩血小板 质量分析 

分 类 号：R457.1[医药卫生—治疗学]