钩枝藤生物碱对斜纹夜蛾幼虫的毒杀活性  

Insecticidal Activity of the Alkaloids from Ancistrocladus tectorius Against the Larvae of Spodoptera litura

作　　者：王军[1] 闫超[2] 蔡彩虹[1] 冯岗[2] 张静[2] 戴好富[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物学与遗传资源利用重点实验室海南省黎药资源天然产物研究与利用重点实验室,海南海口571101 [2]中国热带农业科学院环境与植物保护研究所,海南海口571101

出　　处：《热带作物学报》2015年第9期1650-1654,共5页Chinese Journal of Tropical Crops

基　　金：海南省重大专项(No.ZDZX2013008-4);国家科技支撑计划课题(No.2013BAI11B04);中医药行业科研专项(No.201207002);中央级公益性科研院所基本科研业务费专项资金(No.1630052012014);三亚市院地科技合作项目(No.2013YD81)

摘　　要：探讨钩枝藤总生物碱及3个单体化合物[(1)Ancistrocline,(2)5′-O-demethylhamatintine,(3)N-methylphylline]对斜纹夜蛾幼虫的毒杀活性,旨在开发新型植物源农药。采用浸虫法测定钩枝藤总碱对斜纹夜蛾3龄幼虫的毒杀活性和采用饲料混毒法测定钩枝藤单体化合物对斜纹夜蛾初孵幼虫毒杀活性。结果表明:钩枝藤总碱对斜纹夜蛾3龄幼虫具有较强的毒杀作用,其第3天和第5天的LC50值分别为2.61 mg/m L和0.91 mg/m L。单体化合物对斜纹夜蛾初孵幼虫具有明显的抑制生长发育作用,其中以化合物(2)抑制作用最强,主要表现为化蛹率(23.33%)、羽化率(13.33%)显著低于对照(化蛹率90.00%、羽化率86.67%)。The insecticidal activity of the total alkaloids and its 3 compounds [（1）： Ancistrocline, （2）： 5＇-O- demethylhamatintine, （3）： N-methylphylline] of Ancistrocladus tectorius against the larvae of Spodoptera litura were discussed for the development of new type of botanical pesticides. The toxicity of the total alkaloids to the 3^rd instar larvae of S. litura was determined by insect-dip method. The toxicity of 3 compounds to the larvae of S. litura was tested by diet-incorporation. The results indicated that the total alkaloids of A. tectorius had strong toxic effect on the 3^rd instar larvae and the LC50 values of 3 d and 5 d was 2.61 mg/mL and 0.91 mg/mL respectively. The growth of newly hatched instar larvae of S. litura was also inhibited by the three compounds. The compound （2） had the strongest inhibition effects with pupation rate 23.33% and eclosion rate 13.33%, which was significantly lower than those of control （pupation rate 90.00%, eclosion rate 86.67%）.

关 键 词：钩枝藤 生物碱 斜纹夜蛾 毒杀活性 抑制生长发育 

分 类 号：S481.1[农业科学—农药学]