金防感冒颗粒标准中检测方法的简化研究  

Study on Simplifying Quality Standard for Jinfang Ganmao Granules

作　　者：张鹏 安丽娜 申玉龙 韩桂茹 

机构地区：[1]承德燕峰药业股份有限公司,石家庄050091

出　　处：《中国药品标准》2015年第4期281-285,共5页Drug Standards of China

摘　　要：目的：为提高检测速度，对金防感冒颗粒质量标准进行简化研究。方法：在两块薄层板上鉴别对乙酰氨基酚、防风和绿原酸；采用高效液相色谱法，以乙腈-甲醇旬．1％磷酸（6：4．5：89．5）为流动相，295nm为检测波长，同时测定样品中对乙酰氨基酚与绿原酸的含量。结果：通过方法学考察，绿原酸与乙酰氨基酚的进样量分别在0．09～0．92μg（r=0．9999）、0．95～9．5μg（r=0．9999）与峰面积呈良好的线性关系；平均回收率分别为98．97％和99．76％，其RSD（n=9）分别为1．95％和1．17％；TLC鉴别阴性无干扰。结论：简化的金防感冒颗粒质量标准简便、快捷、实用。Objective： To speed up test time, the simplification on quality standard for Jinfang Ganmao Granules was studyed. Methods： Aeetaminophen, Saposhnikoviae Radix and chlorogenic acid were identified by TLC on 2 thin-layer chromatographic plates. The contents of chlorogenic acid and acetaminophen were determined by HPLC with a mixture of acetonitrile, methanol and 0. 1% phosphoric acid （6： 4. 5： 89. 5 ） as the mobile phase, the detection wavelength was 295 nm. Results： The methodological study showed chlorogenic acid and acetaminophen had a good linear correlation in the range of 0. 09 - 0. 92 μg （ r = 0. 999 9 ） and 0. 95 - 9. 5 μg （ r = 0. 999 9）. The average recovery of chlorogenic acid and acetaminophen was 98. 97% and 99. 76% , respectively ; RSD （n =9） was 1.95% and 1.17%, respectively. The negative samples had no interference on TLC identification. Conclusion： The simplified quality standard of Jinfang Ganmao Granules is simple, rapid and usable.

关 键 词：金防感冒颗粒 高效液相色谱法 绿原酸 对乙酰氨基酚 

分 类 号：R921.2[医药卫生—药学]