多指标加权评分正交试验优选消痤散提取工艺  

Optimization of Extraction Process for Xiao Cuo Powder by Orthogonal Test of Multi-index Weighted Score

作　　者：刘敏[1] 杨全伟[1] 刘新国[1] 徐宏峰[1] 余南才[1] 

机构地区：[1]武汉市第一医院药学部,武汉430022

出　　处：《中西医结合研究》2015年第4期194-197,共4页Research of Integrated Traditional Chinese and Western Medicine

摘　　要：目的优选消痤散提取工艺。方法以加水量、提取次数和煎煮时间为因素进行正交试验,通过高效液相法测定其黄芩苷、芍药苷含量,以黄芩苷和芍药苷提取量和提取干膏收得率进行加权评分。结果消痤散加10倍量水煎煮,每次煎煮1h,共提取3次所得黄芩苷、芍药苷含量较高,干膏收得率较高。结论多指标加权评分正交试验优选消痤散提取工艺为10倍量水煎煮3次,每次煎煮1h。Objective To optimize the extraction process for Xiao Cuo powder. Methods Adding water amount,extraction times and extraction time were used as factors of the orthogonal test and the content of baicalin and paeoniflorin were determined by high performance liquid chromatography（HPLC） method. The extraction rate baicalin and paeoniflorin and the extraction rate of dry extract were weighted by a weighted score. Results The content of baicalin and paeoniflorin were higher by the extraction process of extracted 3 times with 10 times the amount of water and 1 h each time. Conclusion Optimization of extraction process for Xiao Cuo powder by orthogonal test of multi-index weighted score was as following：extracted 3 times with 10 times the amount of water, 1 h each time.

关 键 词：正交试验 多指标加权评分 提取工艺 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]