低温机械灌注对大鼠供肝代谢作用的影响  

Effect of hypothermic machine perfusion on the metabolism of liver in rats

作　　者：李弦[1] 王伟[2] 胡晓燕[1] 王彦峰[1] 熊艳[1] 叶啟发[2] 

机构地区：[1]武汉大学中南医院、武汉大学肝胆疾病研究院,430071 [2]中南大学湘雅三医院、卫生部移植医学工程技术研究中心,长沙

出　　处：《中华肝胆外科杂志》2015年第9期625-628,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金（21334005）;高等学校博士学科点专项科研基金（20100141110016）;湖北省自然科学基金（2012FFA044;2013CFB258）;武汉市科学技术计划（2013060705010326;2013060602010247）;武汉大学医学部协同创新项目

摘　　要：目的探索缺血30min后肝细胞代谢变化及低温机械灌注对肝细胞代谢的影响。方法将40只SD雄性大鼠随机均分为A、B、C、D四组。A组供肝热缺血0min＋冷保存5h；B组热缺血0min＋冷保存4h＋机械灌注1h；C组供肝热缺血30min＋冷保存5h；D组供肝热缺血30min＋玲保存4h＋机械灌注1h。在机械灌注过程中每隔10min记录阻力指数值。完成灌注后切取0．2g肝组织检测肝糖原含量及丙二醛（MDA）值。结果经机械灌注处理后肝糖原含量较冷保存有所降低，但差异无统计学意义（P〉0．05）；A、B两组MDA含量无差异（P〉0．05）；D组MDA含量明显高于c组（P〈0．05）；经机械灌注处理后，肝脏阻力指数值均有明显降低。结论肝脏遭受30min热缺血损伤后，再行机械灌注，可降低其阻力指数，增加肝细胞内细胞代谢，使得脂质过氧化物生成增多。Objective To examine the metabolic variations in liver after 30 min warm ischemic in- jury, and the effect of hypothermic machine perfusion on the metabolism in rats. Methods 40 SD male rats were randomly divided into 4 groups ： Group A, the liver underwent warm ischemia for 0 min and cold stor- age （CS） for 5 h; Group B, the liver was treated by warm ischemia for 0 min, CS for 4 h and then machine perfusion （MP） for 1 h; Group C, the liver suffered from warm isehemia for 30 min and CS for 5 h; and Group D, the liver was treated by warm ischemia for 30 min plus CS for 4 h plus MP for 1 h. During the MP process, the perfusion resistance index was recorded every 10 min, and the liver glycogen content and ma- londialdehyde （MDA） value were also detected. Results The hepatic glycogen content decreased after MP treatment, but there was no statistical significance （ P 〉 0. 05 ）. No differences on MDA contents was found between Group A and B （ P 〉 0. 05 ） , while MDA in Group D was significantly higher than that in Group C （P 〈 0. 05 ）. After hypothermic MP treatment, the liver resistance index value was significantly reduced. Conclusion MP could reduce the resistance index but increase metabolic rate in liver undergoing warm ischemic iniury, thus producing more lipid peroxides.

关 键 词：低温机械灌注 肝糖原 阻力指数 丙二醛 供肝 大鼠 

分 类 号：R589.2[医药卫生—内分泌]