通气量对灵芝菌丝体液态深层发酵合成灵芝三萜的影响  

Effects of Aeration Rate on the Production of Triterpenes by Ganoderma lucidum G0119 in Liquid Submerged Fermentation

作　　者：冯杰 冯娜 杨焱 刘方 贾薇 张劲松 

机构地区：[1]国家食用菌工程技术研究中心农业部南方食用菌资源利用重点实验室上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所,上海201403

出　　处：《天然产物研究与开发》2015年第9期1564-1570,共7页Natural Product Research and Development

基　　金：上海市市级农口系统青年人才成长计划资助项目(2015-1-7);上海市农业科学院青年科技人员"助跑"计划(ZP06)

摘　　要：采用5 L搅拌式发酵罐研究灵芝菌丝体液态深层发酵合成灵芝三萜的扩大培养条件,考察了不同条件下的通气量对灵芝菌丝体生长、比生长速率、灵芝三萜合成、比合成速率、还原糖消耗、还原糖比消耗速率、铵根离子消耗以及铵根离子比消耗速率的影响。研究结果表明,通气量能够显著影响菌丝体的生长以及灵芝三萜的合成,当通气量为8 L/min时,菌丝体合成灵芝三萜得率和生产强度最高,分别为0.204 g/L和0.00131 g/(L·h),最大菌丝体得率达到8.77 g/L。发酵过程中菌丝体最大比生长速率为0.0704 1/h,灵芝三萜最大比合成速率为0.0256 1/h,还原糖和铵根离子最大比消耗速率分别为0.562 1/h和0.0171 1/h。The effects of aeration rate on the mycelial growth of Ganoderma lucidum GOl19, specific mycelial growth rate, triterpenes production, specific triterpenes production rate, residual sugar consumption, specific residual sugar con- sumption rate, ammonium consumption and specific ammonium consumption rate were investigated in four parallel 5 L batch fermenters. The results revealed that the aeration rate had significant impacts on the growth of G. luc/dum G0119 and production of triterpenes. The yield and productivity of triterpenes were the highest when aeration rate was 8 L/min, reached 0.204 g/L and 0.00131 g/（ L · h） ,respectively. The maximum yield of the mycelia reached 8.77 g/L under 8 L/min at the end of the fermentation. The maximum specific mycelial growth rate, maximum specific triterpenes produc- tion rate,maximum specific residual sugar consumption rate and maximum specific ammonium consumption rate reached 0. 0704,0. 0256,0. 562 1/h and 0.0171 1/h during the fermentation.

关 键 词：灵芝 菌丝体 通气量 液态深层发酵 灵芝三萜 

分 类 号：Q815[生物学—生物工程]