hTERC基因在预测宫颈上皮内瘤变Ⅰ进展和转归中的价值  

The Value of hTERC Gene Detection in Predicting Progression and Regression of Cervical Intraepithelial Neoplasia Grade Ⅰ

作　　者：孙佳星[1,2] 王莹[2] 肖琳[2] 米贤军[2] 罗小婉[2] 栾峰[2] 符丽华[2] 陈昂[2] 李玉鹏[1,2] 

机构地区：[1]南方医科大学第三临床医学院,广东广州510630 [2]南方医科大学附属中山博爱医院,广东中山528400

出　　处：《实用妇产科杂志》2015年第9期680-683,共4页Journal of Practical Obstetrics and Gynecology

基　　金：国家科技部"十一五"重点支撑项目(编号:2008BAI57B01);广东省科技计划项目(编号:2012B031800023)

摘　　要：目的:探讨荧光原位杂交(FISH)技术检测宫颈石蜡包埋切片中人类染色体端粒酶RNA(h TERC)基因扩增情况,在预测宫颈上皮内瘤变(CIN)Ⅰ进展和转归中的价值。方法:收集55例CINⅠ患者的宫颈石蜡包埋病理标本,随访后根据病理学确诊情况分为3组,CINⅡ、Ⅲ及宫颈癌组(进展组)13例、CINⅠ组(持续组)19例、正常或炎症组(转归组)23例。采用FISH技术检测不同组中宫颈石蜡包埋切片中h TERC基因扩增情况。结果:共有54例CINⅠ患者的宫颈石蜡包埋病理标本纳入研究,h TERC基因扩增阳性22例,其中进展组12例,持续组9例,转归组1例,阳性率分别为进展组100.00%,持续组47.37%,转归组4.35%。3组h TERC基因扩增阳性率比较差异有统计学意义(P<0.01)。异常信号比2∶3、2∶4、4∶4和N∶≥5的细胞数比例3组比较,差异有统计学意义(P<0.01)。结论:采用FISH技术检测宫颈石蜡包埋切片中h TERC基因扩增情况可以预测CINⅠ的进展和转归;异常信号比为4∶4和N∶≥5可能可以用来预测CINⅠ的进展。Objective：To investigate the expression of human telomerase RNA component（hTERC）gene inparaffin-embedded cervical tissue specimens and its clinical application for predicting progression and regression of cervical intraepithelial neoplasia grade I. Methods： According to the pathological diagnosis, cervical paraf- fin-embedded pathological specimen from 55 cases of CIN type I patients were divided into three groups. 13 patients progressed from CIN I to CIN Ⅱ/Ⅲ or cervical cancer（Group1 ） ,19 patients persisted with CIN Ⅱ （Group 2）and 23 patients regressed （Group 3）. The hTERC gene was detected by fluorescence in situ hybridization （FISH）. Results：54 from 55 paraffin-embedded cervical specimens with CIN I were enrolled. 22 cases were positive of hTERC gene amplification,of which 12 cases progressed,9 cases persisted with CIN 1 and 1 patients regressed spontaneously. The positive rates of hTERC gene amplification in patients were 100.00% （12/12）, 47. 37% （9/19）and 4. 35% （1/23） respectively,with a significant difference among three groups. The number of cells with signal ratio pattern of 2 ： 3,2 ： 4,4 ： 4 and N ： ≥5 in three groups was significantly different. Conclu- clone：The detection of hTERC amplification in paraffin-embedded cervical tissue specimens by FISH could predict the progression and regression of CIN I . The signal ratio pattern of 4 ： 4 or N ： ≥5 may indicate the progres- sion of CIN I.

关 键 词：宫颈癌 宫颈上皮内瘤变 人类染色体端粒酶RNA基因 荧光原位杂交技术 石蜡包埋切片 

分 类 号：R713.4[医药卫生—妇产科学]