不同浓度的血清素对体外培养大鼠成骨细胞分化的影响  

The effects of serotonin at different concentrations on differentiation of cultured primary rat osteoblasts in vitro

作　　者：刘海波[1] 孙海飚[2] 韩晓强[2] 卢向东[2] 韩树峰[2] 王彤[3] 

机构地区：[1]山西医科大学第一临床医学院,太原030001 [2]山西医科大学第一医院骨科,太原030001 [3]山西医科大学公共卫生学院,太原030001

出　　处：《世界复合医学》2015年第3期227-230,共4页World Journal of Complex Medicine

基　　金：山西省自然科学基金(2013011057-3)

摘　　要：目的:研究不同浓度的血清素对体外培养大鼠成骨细胞分化的影响。方法:分离培养新生SD大鼠颅骨的成骨细胞,分别向培养液中加入不同浓度的血清素,分为4组:0 mol/L组(空白对照组)、10-9 mol/L组、10-8 mol/L组、10-7 mol/L组,通过RT-PCR检测骨钙素(OCN),碱性磷酸酶(ALP),Runt相关转录因子-2(Runx 2)和Osterix m RNA相对表达量的变化。结果:不同浓度的血清素均可以抑制骨钙素(OCN),碱性磷酸酶(ALP),Runt相关转录因子-2(Runx 2)和Osterix m RNA的相对表达量,且这种抑制作用随着血清素浓度的增大而增强。结论:血清素可以剂量依赖性地抑制成骨细胞的分化,这为骨质疏松症的治疗提供了新的思路。Objective： to study the effects of serotonin at different concentrations on differentiation and of cultured primary rat osteoblasts in vitro. Methods： the osteoblasts from the skull of newborn SD rats, were divied into four groups by the culture medium with different serotonin concentrations： 0mol/L group（blank control）, 10-9mol/L group, 10-8mol/Lgroup and 10-7mol/L group. In addition, mRNA expression of osteocalcin（OCN）, alkaline phosphatase（ALP）, runt-related transcription factor2 （Runx2） and Osterix were quantitated by real-time reverse transcription polymerase chain reaction. Results： different concentrations of serotonin inhibited the mRNA expression of OCN, ALP, Runx2and Osterix of osteoblasts; And, the inhibitory effect of serotonin was dose-dependent. Conclusion：serotonin can inhibit differentiation of osteoblasts; which provide a novel approach for the treatments of osteoporosis.

关 键 词：血清素 成骨细胞 分化 

分 类 号：R681[医药卫生—骨科学]