An Improved Turn-On Aptasensor for Thrombin Detection Using Split Aptamer Fragments and Graphene Oxide  

作　　者：Xingfen Liu Yonghong Yang Xiaoxiao Hua Xiaomiao Feng Shao Su Yanqin Huang Quli Fan Lianhui Wang Wei Huang 

机构地区：[1]Key Laboratory for Organic Electronics & Information Displays and Institute of Advanced Materials, NationalJiangsu Synergetie Innovation Center for Advanced Materials, Nanjing University of Posts & Telecommunications, Nanjing, Jiangsu 210023, China [2]Key Laboratory of Flexible Electronics & Institute of Advanced Materials, National Jiangsu Synergetic Innovation Center for Advanced Materials, Nanjing Teeh University, Nanj ing, Jiangsu 211816, China

出　　处：《Chinese Journal of Chemistry》2015年第8期981-986,共6页中国化学（英文版）

摘　　要：An improved tum-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide （GO） was reported. The thrombin-binding aptamer （Aptl5） was split into two parts for target recognition, an 8-base se- quence labeled with fluorescein （FAM-Apt-A） and a 7-base oligonucleotide sequence （Apt-B）. In the absence of target protein, the fluorescence of FAM-Apt-A/Apt-B was quenched by GO through n-n stacking between GO and single-stranded DNA. However, when thrombin was introduced into the system, a target-induced G-quadruplex forms with two split aptamer fragments and thrombin. The fluorescence recovered due to weak interaction between G-quadruplex and GO. Compared to the strategy using intact aptamer, probe concentration was lowered, and an improved sensitivity was obtained. Moreover, heating process to avoid unfavorable secondary structure was avoided due to the use of shorter split aptamer fragments.An improved tum-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide （GO） was reported. The thrombin-binding aptamer （Aptl5） was split into two parts for target recognition, an 8-base se- quence labeled with fluorescein （FAM-Apt-A） and a 7-base oligonucleotide sequence （Apt-B）. In the absence of target protein, the fluorescence of FAM-Apt-A/Apt-B was quenched by GO through n-n stacking between GO and single-stranded DNA. However, when thrombin was introduced into the system, a target-induced G-quadruplex forms with two split aptamer fragments and thrombin. The fluorescence recovered due to weak interaction between G-quadruplex and GO. Compared to the strategy using intact aptamer, probe concentration was lowered, and an improved sensitivity was obtained. Moreover, heating process to avoid unfavorable secondary structure was avoided due to the use of shorter split aptamer fragments.

关 键 词：sensor split aptamer graphene oxide 

分 类 号：O571.421[理学—粒子物理与原子核物理] U472.43[理学—物理]