不同激活剂对油藏内源微生物群落结构的影响  

Effect of different activator systems on oil reservoir indigenous microbial community structure

作　　者：任国领[1] 徐晶雪[1] 张莹[2] 曲丽娜[1] 乐建君[3] 张虹[1,2] 黄永红[1] 

机构地区：[1]大庆师范学院生物工程学院,黑龙江大庆163712 [2]东北师范大学生命科学学院,吉林长春130024 [3]大庆油田有限责任公司勘探开发研究院,黑龙江大庆163712

出　　处：《中国微生态学杂志》2015年第9期1004-1008,共5页Chinese Journal of Microecology

基　　金：黑龙江省青年科学基金项目(QC2011094);黑龙江省教育厅科学技术研究项目(12513005);大庆师范学院博士启动基金(12ZR06)

摘　　要：目的筛选大庆油田聚驱后油藏内源微生物采油激活剂的类型。方法在动态物理模拟实验模型基础上,利用基于16S rDNA的基因克隆文库方法,分析了两组激活剂配方激活的微生物群落结构。结果配方1激活的微生物菌群主要有假单胞菌属(75%)、深海弯曲菌属(18%)、陶厄菌属(6%);配方2激活的微生物菌群主要有陶厄菌属(50%)、假单胞菌属(26%)、螺杆菌属(15%)、梭菌属(9%)。结论激活剂配方2更适合大庆油田聚驱后油藏内源微生物采油。Objective To screen the activator type of indigenous microorganism flooding of oil reservoir after polymer flooding in Daqing oilfield. Methods The structure of microbial community activated by two activator systems was analyzed with a method based on 16 S rDNA gene clone library on the basis of dynamic physical experiment model. Results The major microorganisms activated by the first activator system were Pseudomonas sp.（ 75%）,Thalassolituus sp.（ 18%） and Thauera sp.（ 6%）. The major microorganisms activated by the second activator system were Thauera sp.（ 50%）,Pseudomonas sp.（ 26%）,Helicobacter sp.（ 15%） and Clostridium sp.（ 9%）. Conclusion The first activator system is more suitable for indigenous microorganism flooding of oil reservoirs after polymer flooding in Daqing oilfield.

关 键 词：聚驱后油藏 激活剂 内源微生物 群落结构 基因克隆文库 

分 类 号：TE357.9[石油与天然气工程—油气田开发工程]