五味子果实不同部位中木脂素成分的分布规律  

Distribution of Lignans in Different Parts of Schisandrae Chinensis Fructus

作　　者：王志明[1] 刘久石 郭耀杰[2] 刘海涛[2] 高石曼 张晶[1] 张本刚[2] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]中国医学科学院,北京协和医学院药用植物研究所,北京100193

出　　处：《中国实验方剂学杂志》2015年第18期35-39,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中央级公益性科研院所基本科研业务专项(YZ-13-18)

摘　　要：目的：建立同时测定五味子果实中10种木脂素类成分含量的方法,分析木脂素类成分在五味子果实的种仁、种皮和果肉中的含量差异,探索各成分的分布规律。方法：采用高效液相色谱法（HPLC）测定不同产地五味子果实中五味子醇甲,戈米辛D,戈米辛J,五味子醇乙,当归酰戈米辛H,戈米辛G,五味子酯甲,五味子甲素,五味子乙素,五味子丙素的含量,流动相乙腈（A）-水（B）梯度洗脱（0-30 min,40%A;30-32 min,40%-46%A;32-53 min,46%A;53-64 min,46%-50%A;64-83 min,50%-57%A;83-107 min,57%-62%A;107-115 min,62%-100%A;115-120 min,100%A）,检测波长220nm,进样量4μL。结果：不同产地五味子果实的种仁、种皮和果肉中总木脂素质量分数分别为30.08,9.76,2.24 mg·g-1。10种木脂素类成分在五味子果实3个不同部位中的含量差异极显著,排序为种仁〉种皮〉果肉。结论：建立的含量测定方法能较好地反映五味子果实的不同部位中木脂素类成分的分布信息,为指导五味子果实的临床合理应用提供参考。Objective： To establish a method for determination of ten lignans in Schisandrae Chinensis Fructus, and analyze difference of contents of ten lignans in the pulp, seed shell and seed kernel of Schisandrae Chinensis Fructus for researching regularities of their distribution. Method ： HPLC was developed for determination of schisandrol A, gomisin D, gomisin J, schisandrol B, angeloylgomisin H, gomisin G, schisantherin A, schisandrin A, sehisandrin B and schisandrin C in Schisandrae Chinensis Fructus from different area, mobile phase consisted of acetonitrile-water for gradient elution, detection wavelength was set at 220 nm and injection volume was 4 μL. Result： The content of total lignans in the seed kernel, seed shell and pulp of Schisandrae Chinensis Fructus from different area were 30.08, 9.76, 2.24 mg ·g^-1, respectively. Contents of ten lignans in these three parts of Schisandrae Chinensis Fructus showed significant difference, which decreased in the order of the seed kernel 〉 the seed shell 〉 the pulp. Conclusion ： The developed method displays distribution information of lignans in three parts of Schisandrae Chinensis Fructus well, which can provide a reference for clinical application of Schisandrae Chinensis Fructus.

关 键 词：五味子 木脂素类成分 分布规律 戈米辛D 戈米辛J 五味子醇乙 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]