一测多评法测定手参中不同类型成分的含量  

Determination of Five Components in Gymnadeniae Conpseae Rhizoma Based on QAMS

作　　者：徐晓雪[1] 李晓鹏[1] 韩伟健[1] 郭娜[1] 林晓彤[1] 钟旭[1] 许枬[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2015年第18期40-43,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技支撑计划项目(2012BAI28B00)

摘　　要：目的:建立手参中熊果苷,天麻素,4-羟基苯甲醇,militarine,loroglossin的一测多评含量测定方法,为手参的质量控制提供依据。方法:采用高效液相色谱法,以天麻素作为内参物,建立与其他4个指标成分间的相对校正因子(RCF),并利用校正因子对熊果苷,4-羟基苯甲醇,militarine,loroglossin的含量进行计算,实现一测多评(计算法);同时采用外标法测定10批手参中上述5种成分的含量,并比较计算值与测定值之间的差异,验证一测多评的合理性、可行性和重复性。结果:天麻素对熊果苷,4-羟基苯甲醇,militarine,loroglossin的相对校正因子分别为3.083 1(2.0%),1.562 2(2.1%),1.545 0(1.7%),1.742 5(1.9%);采用相对校正因子计算10批手参药材中5种成分的计算值与测定值的RSD均<3%,表明两者无显著差异。结论:建立的一测多评法准确可行,可以用于同时测定手参中天麻素,militarine,loroglossin等5种成分的含量。Objective： To develop a quantitative analysis of multi-components by single-marker （QAMS） method for the simultaneous determination of five components （gastrodin, militarine, loroglossin, arbutin, 4-hydroxybenzenemethanol） in Gymnadeniae Conpseae Rhizoma, in order to provide basis for the quality control. Method： Gastrodin was used as the internal reference substance, and the relative correlation factors （RCF） with militarine, loroglossin, arbutin and 4-hydroxybenzenemethanol were determined by HPLC-UV. The contents of the four components were calculated according to the RCF, respectively. The contents of these components in 10 batches of Gymnadeniae Conpseae Rhizoma were determined by the external standard method. The rationality, feasibility, and repeatability of the QAMS method were verified by comparing the results obtained from the two different methods. Result： RCF of gastrodin-arbutin, gastrodin-4-hydroxybenzenemethanol, gastrodin-militarine and gastrodinloroglossin were 3. 083 1 （2.0%）, 1. 562 2 （2.1%） , 1. 545 0 （ 1.7% ）, 1. 742 5 （1.9%）, respectively. For the five components, there was no significant difference between the calculated and determined values RSD （ 〈 3% ） of the 10 batches by the two different methods. Conclusion： The method established in this study is accurate and feasible and can be used for the determination of five components in Gymnadeniae Conpseae Rhizoma simultaneously.

关 键 词：手参 一测多评 相对校正因子 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]