清络通痹方与精简方的药效学比较  

Comparison on Pharmacodynamics Effects of Qingluo Tongbi Prescription and Its Simplified Prescription

作　　者：朱亚梅[1] 周玲玲[1] 袁玉丽[1] 周聪[1] 刘慧[1] 柳璋璞[1] 陈雪[1] 周学平[1] 

机构地区：[1]南京中医药大学,南京210023

出　　处：《中国实验方剂学杂志》2015年第18期99-103,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81072749);2014江苏省普通高校研究生科研创新计划项目(SJZZ_0122)

摘　　要：目的:探讨清络通痹方精简前后的药效学作用及影响机制。方法:DBA/1小鼠,复制胶原性关节炎(CIA)模型,随机分为空白组,模型组,清络通痹精简方组(8.4 g·kg-1),清络通痹原方组(13 g·kg-1)及雷公藤多苷片组(9 mg·kg-1),观察一般情况及关节肿胀,ig给药4周后,检测血清中白介素-1β(IL-1β),肿瘤坏死因子-α(TNF-α),抗Ⅱ型胶原抗体(抗CⅡ抗体)含量;踝关节HE染色观察病理学改变及病理评分。结果:与正常组比较,模型组小鼠关节肿胀明显,关节炎评分,TNF-α,IL-1β和抗CⅡ抗体水平明显升高(P<0.01);与模型组比较,清络通痹精简方及原方、雷公藤多苷均能明显抑制小鼠足肿胀,降低关节炎评分,降低血清中TNF-α,IL-1β和抗CⅡ抗体水平(P<0.01,P<0.05);病理学检查提示可减轻关节病理损伤,关节总病理评分降低(P<0.01)。结论:清络通痹精简方将不同性味、不同功效药物合理配伍,治疗效果和作用机制与原方相似。Objective： To observe pharmacodynamic effects and mechanism of simplified Qingluo Tongbi prescription （SQLT）. Method： The model of rheumatoid arthritis was induced by collagen Ⅱ in DBA/1 mice. The mice were randomly divided into blank group, collagen-induced arthritis （CIA） group, SQLT group （8.4 g ·kg^-1） , original Qingluo Tongbi prescription （QLT） group （13 g ·kg^-1） and polyglucosides of Triplegium wilfordii （T Ⅱ ） group （9 mg ·kg^-1）. General conditions and joint swelling were observed. All groups were treated with corresponding drugs through the intragastric administration for four weeks. Interleukin-1β （IL-1β） , tumor necrosis factor-α （TNF-α） , anti collagen Ⅱ （C Ⅱ ） antibody in serum were measured, and hematoxylin-eosin （HE） staining was conducted to observe the histopathologic changes and pathological score of ankle tissues. Result： Compared with the normal group, the model group showed significant joint swelling and increases in TNF-α, IL-1β and anti C Ⅱ antibody, with high ankle osteoarthritis scores （P 〈0.01 ）. Compared with the CIA group, QLT, SQLT and T Ⅱ groups showed notable reductions in the paws swelling, arthritis scores, contents of IL-1β, TNF-α and anti C Ⅱ antibody in serum （P 〈0.01, P 〈 0.05）, and inhibition in the histological damage and cumulative arthritis injury scores. Conclusion： SQLT rationally integrates herbs with different properties, tastes and efficacies, with a similar effect and mechanism with the original prescription.

关 键 词：清络通痹方 处方精简 胶原性关节炎小鼠 药效比较 

分 类 号：R285.5[医药卫生—中药学]