肾康注射液对腹膜透析液诱导的大鼠腹膜组织形态及TNF-α,TGF-β_1的影响  

Effect of SKI on Peritoneal Morphology Changes Induced by Peritoneal Dialysis Solution and TNF-α and TGF-β_1 in Peritoneal Dialysis Rats

作　　者：董莉[1] 程世平[2] 查艳[2] 袁静[2] 沈燕[2] 林鑫[2] 

机构地区：[1]贵阳医学院贵州省药物制剂重点实验室,贵阳550004 [2]贵州省人民医院,贵阳550004

出　　处：《中国实验方剂学杂志》2015年第18期130-133,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科学技术基金项目(黔科合J字[2011]2252号)

摘　　要：目的:研究肾康注射液(Shenkang injection,SKI)对腹膜透析液(peritoneal dialysis solution,PDS)诱导的大鼠腹膜组织形态及肿瘤坏死因子-α(TNF-α),转化生长因子-β1(TGF-β1)影响。方法:50只SD大鼠,随机分为正常组,4.25%PDS组和SKI低、中、高剂量组;除正常组不注射PDS外,其余4组分别ip 4.25%PDS,低、中、高剂量的SKI+4.25%PDS。于注射8周后,水合氯醛麻醉各组大鼠,同时沿腹白线剪开腹壁,量取腹腔内的透出液,ELISA法测定透出液中TNF-α的含量。取壁层腹膜组织,观察腹膜组织形态改变,并用免疫组化法检测TGF-β1的表达。结果:与正常组比较,PDS组大鼠透出液中TNF-α含量和壁层腹膜间皮细胞的TGF-β1表达显著升高(P<0.01),且腹膜间皮细胞重度肿胀变性、脱落,间皮下基质明显增生,并可见大量血管增生及炎细胞浸润。与PDS组比较,SKI中、高剂量组的TNF-α含量显著降低(P<0.01),SKI各剂量组壁层腹膜间皮细胞TGF-β1的表达明显减少(P<0.05),且腹膜结构改变减轻、炎症细胞浸润和基质增生减少。结论:腹膜透析液中加入SKI具有改善PDS诱导腹膜纤维化,其作用机制可能与抑制TNF-α,TGF-β1的增加有关。Objective： To investigate the effect of Shenkang injection （SKI） on the morphology changes induced by peritoneal dialysis solution （PDS） as well as tumor necrosis factor-α （TNF-α） and transforming growth factor-β1 （TGF-β1） in peritoneal dialysis rats. Method： Fifty SD rats were randomly divided into normal group, 4.25% PDS group, 4.25% SKI low, middle and high dose groups. Except for the normal group, all of the remaining four groups were intraperitoneally injected with low, middle and high doses of SKI ＋ 4.25% PDS. At the 8^th week after the injection, rats were anesthetized with chloral hydrate, and the peritoneal solution was gathered. The level of TNF-α was detected by ELISA method. The visceral peritoneum tissues of rats were stained with hematoxylin-eosin （HE） to observe the changes of peritoneal morphology. Immunohistochemistry were used to analyze the expression of TGF-β1. Result： Compared with the normal group, rats in the PDS group showed significantly higher TNF-α and expressions of TGF-β1 （P 〈 0. 01 ） , swellon and deciduous peritoneal mesothelial cells, thickened sub-mesothelial matrix, and severe vascular proliferation and inflammatory cell infiltration. Compared with the PDS group, SKI middle and high dose groups showed decrease in TNF-α content （P 〈 0.01 ）. all SKI groups showed great reliefs in the structure changes of the peritoneum, decrease in the inflammatory cell infiltration and expression of TGF-β1 （P 〈 0.05, P 〈 0.01 ）. Conclusion： SKI may be helpful in ameliorating the peritoneal fibrosis induced by PDS, which ,nay be correlated with inhibiting increases in TNF-α and TNF-β1.

关 键 词：肾康注射液 腹膜纤维化 肿瘤坏死因子-α 转化生长因子-Β1 

分 类 号：R285.5[医药卫生—中药学]