多普勒彩超辐照对不同孕期胎鼠脑组织超微结构的影响  

Effects of color Doppler ultrasound insonification on brain ultrastructure of fetal rats from different maternal pregnancy periods

作　　者：朱海燕 袁青宁 杨勇骥[2] 

机构地区：[1]山东医学高等专科学校附属医院超声科,临沂276004 [2]第二军医大学基础部生物物理学教研室,上海200433

出　　处：《第二军医大学学报》2015年第9期1025-1028,共4页Academic Journal of Second Military Medical University

摘　　要：目的采用多普勒彩超辐照不同孕期大鼠,观察超声对胎鼠脑组织超微结构的影响。方法采用多普勒彩超照射不同孕期大鼠(孕12d、15d、18d)30min,仪器采用PREIRUS型彩色电脑声像仪,照射条件:EUP-LTM4探头,二维频率5.0 MHz,组织热指数(Tis)=0.4,机械指数(Micd)=1.2。照射后24h取胚胎脑组织标本,利用透射电镜(TEM)观察细胞器超微结构变化。结果经过多普勒彩超辐照30min后,孕期12d胎鼠神经元细胞器超微结构改变不明显,偶见线粒体嵴溶解,内质网未见明显扩张,细胞核形状较规则,胞质中有较小的空泡出现;孕期15d胎鼠神经元细胞器超微结构改变明显,细胞器损伤程度重于12d胎鼠;孕期18d胎鼠可见明显超微结构改变,内质网明显扩张,细胞核形态严重不规则,线粒体嵴溶解更明显,核周间隙扩张,胞质中出现较大空泡。结论多普勒彩超辐照对不同孕期胎鼠脑组织中细胞器超微结构的影响不同,胎鼠孕期越早,相同的辐照对其影响越小。Objective To study the effects of color Doppler ultrasound insonification of maternal rats of different pregnancy periods on the brain ultrastructure of fetal rats. Methods SD rats of different pregnant periods （12 d, 15 d, and 18 d） were insonificated by color Doppler ultrasound for 30 min. The PREIRUS color computer audio-visual device was used with the insonificating parameters as follows： EUP-LTM4, HS. 0 MHz, Tis=0. 4, and Micd~ 1.2. The fetal brains of the each group was collected 24 hours after insonification, and the changes of brain ultrastructure were observed by transmission electron microscope （TEM）. Results There were no significant changes on brain ultrastructure in fetal rats of 12-day maternal pregnancy, with occasional dissolved mitochondria cristae, slight expansion of endoplasmic reticulum, relatively regular nucleus shape and smaller vacuoles in the cytoplasm. There were obvious changes on brain ultrastructure in fetal rats of 15-day maternal pregnancy, with the injury of organelles being severer than that of 12-day maternal pregnancy. There were significant changes on brain ultrastructure in fetal rats of 18-day maternal pregnancy, with obvious expansion of endoplasmic reticulum, severely irregular nuclear shape, obviously dissolved mitochondrial cristae, expansive nuclear gap and large vacuoles in the cytoplasm. Conclusion Color Doppler ultrasound insonification at different maternal pregnancy periods have different effects on the brain ultrastrueture of fetal rats; the earlier the pregnancy period, the smaller the effects on brain ultrastructure of fetal rats.

关 键 词：胚胎发育 彩色多普勒超声检查 脑 超微结构 

分 类 号：R358.1[医药卫生—基础医学]