HPLC法同时测定慢性盆腔炎灌肠液中穿心莲内酯和脱水穿心莲内酯的含量  

Determination of andrographolide and dehydroandrographolide in Chronic Pelvic Inflammatory Disease Enema by HPLC

作　　者：陈龙浩[1] 黄涛阳[1] 孙春玲[1] 叶力通[1] 晏聪慧[1] 杨日娟 

机构地区：[1]广州军区广州总医院药学部,广州510010

出　　处：《西北药学杂志》2015年第5期575-577,共3页Northwest Pharmaceutical Journal

基　　金：广东医院制剂研究开发技术平台建设(编号:2011A080300004)

摘　　要：目的建立用反相高效液相色谱法测定慢性盆腔炎灌肠液中穿心莲内酯和脱水穿心莲内酯含量的方法。方法色谱柱：Agielnt TC-C18（250mm×4.6mm,5μm）;流动相：甲醇-水（52∶48）;柱温：不设定;流速：1.0mL·min^-1;检测波长：225nm（穿心莲内酯）,254nm（脱水穿心莲内酯）。结果穿心莲内酯和脱水穿心莲内酯的回归方程为：A=22 274 C-49.395,r=0.999 8;A=15 904 C-3.691,r=0.999 6;线性范围分别为：0.020 58-0.205 8和0.008 328-0.083 28mg·mL^-1。平均回收率分别为99.62%和100.12%;RSD分别为1.81%和2.05%。结论 HPLC法测定慢性盆腔炎灌肠液中穿心莲内酯和脱水穿心莲内酯的含量,具有专属性强、精密度好、操作简单、准确度高等特点。Objective To establish a method for determining the content of andrographolide and dehydroandrographolide in Chronic Pelvic Inflammatory Disease Enema by RP‐HPLC .Methods Agilent TC‐C18column（250 mm × 4 .6 mm ,5 μm） was used ,mobile phase was methanol‐water（52∶48） ,and the flow rate was 1 .0 mL · min^-1 .The detection wavelength was 225 nm for androgra‐pholide and 254 nm for dehydroandrographolide .Results The regression equations for andrographolide and dehydroandrographol‐ide were：A=22 274 C—49 .395 ,（r=0 .999 8） and A=15 904 C—3 .691 ,（r=0 .999 6） ,respectively .The linear ranges were 0 .020 58‐0 .205 8 and 0 .008 328‐0 .083 28 mg · mL ^-1 ,respectively .The average recoveries（RSD） were 99 .6% （1 .81% ） ,100 .1%（2 .05% ） ,respectively .Conclusion The method is rapid ,accurate and reliable .

关 键 词：慢性盆腔炎灌肠液 穿心莲内酯 脱水穿心莲内酯 HPLC 

分 类 号：R284[医药卫生—中药学]