成人特发性血小板减少性紫癜患者外周血γδT细胞及IL-17、IL-23水平的变化和意义  

Changes and Significance of Peripheral Blood γδT Cells and IL-17 and IL-23 Levels in Patients With Idiopathic Thrombocytopenic Purpura

作　　者：杨国雷[1] 张复华[1] 凌奕文[1] 牛国敏[1] 

机构地区：[1]南方医科大学附属南海医院,佛山528200

出　　处：《中国继续医学教育》2015年第24期41-43,共3页China Continuing Medical Education

摘　　要：目的通过检测ITP患者外周血γδT细胞、IL-17与IL-23水平的变化,进一步探讨ITP的发病机制,为ITP的防治新途径提供理论依据。方法采用流式细胞术检测外周血γδT细胞;酶联免疫吸附试验方法检测IL-17、IL-23。实验组分正常对照组和ITP组;ITP组分为治疗前组与治疗后组(有效组、无效组);ITP组初始均予地塞米松10 mg/d,此后口服强的松维持并逐渐减量,总疗程1个月。结果正常对照组外周血γδT、IL-17、IL-23分别为(6.41±1.76)%,(43.04±6.10)pg/ml,(34.03±3.64)pg/ml;ITP治疗前组:γδT、IL-17、IL-23分别为(14.90±2.41)%,(76.67±11.86)pg/ml,(63.06±12.08)pg/ml;治疗有效组:γδT、IL-17、IL-23分别为(7.19±1.29)%,(53.95±11.97)pg/ml,(37.12±3.89)pg/ml;治疗无效组:(12.87±2.11)%,(62.51±10.11)pg/ml,(58.42±10.08)pg/ml。结论 ITP患者外周血γδT细胞、IL-17、IL-23水平较正常人高,γδT细胞、IL-17、IL-23参与了ITP的发生发展;ITP患者治疗有效者γδT细胞、IL-17、IL-23水平下降。Objective To explore the pathogenesis of idiopathic thrombocytopenic purpura （ITP）, and provide theoretical basis for new pathways to the prevention and control of ITP, through testing γδT cells, IL-17 and IL-23 in peripheral blood of ITP patients. Methods γδT cells in peripheral blood was testing by flow cytometry. IL-17 and IL-23 in peripheral blood was testing by enzyme-linked immunosorbent assay. Experiment group was divided into normal control group and ITP patient group. ITP patient group was divided into pre-treatment group and＆amp;nbsp;treatment group （effective and non-effective group）. ITP patient group was initially given dexamethasone 10 mg/d, following oral prednisone （reduced gradually）, total course lasting a month. ResultsγδT cells, IL-17 and IL-23 in peripheral blood of normal control group were （6.41±1.76）%, （43.04± 6.10） pg/ml, （34.03±3.64） pg/ml, respectively.γδT cells, IL-17 and IL-23 in peripheral blood of ITP pre-treatment group were （14.90±2.41）%, （76.67±11.86） pg/ml, （63.06±12.08） pg/ml, respectively.γδT cel s, IL-17 and IL-23 in peripheral blood of ITP effective treatment group were （7.19±1.29）%, （53.95±11.97） pg/ml, （37.12±3.89） pg/ml, respectively. γδT cells, IL-17 and IL-23 in peripheral blood of ITP non-effective treatment group were （12.87±2.11%）%, （62.51±10.11） pg/ml, （58.42±10.08） pg/ml. respectively. Conclusion The level ofγδT cells, IL-17 and IL-23 in peripheral blood of ITP patient was higher than that in normal peoplem, therefore they play a part in the development of ITP. After effective treatment, the level of γδT cells, IL-17 and IL-23 in peripheral blood of ITP patient declined.

关 键 词：ITP ΓΔT细胞 白介素-17 白介素-23 

分 类 号：R593.2[医药卫生—内科学]