HPLC法同时测定胎盘灌流液中双黄连冻干粉中8种成分的含量  

Simultaneous Determination of the Contents of 8 Ingredients of Shuanghuanglian Freeze-dried Powder in Placental Perfusate

作　　者：赵丽颖[1] 宋殿荣[2] 

机构地区：[1]天津中医药大学研究生院,天津300193 [2]天津中医药大学第二附属医院妇科,天津300150

出　　处：《中国药房》2015年第27期3823-3825,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(No.81273938)

摘　　要：目的：建立同时测定胎盘灌流液中双黄连冻干粉中8种成分含量的方法。方法：采用高效液相色谱法。色谱柱为Zorbax-C18,流动相为乙腈-1%甲酸水溶液（梯度洗脱）,内标为葛根素,流速为1.0 ml/min,检测波长为280 nm,柱温为25℃,进样量为10μl。结果：8种成分在各自的质量浓度范围内与峰面积呈良好的线性关系（r≥0.999 0）;日内精密度、日间精密度、重复性试验的RSD≤1.9%,稳定性试验的RSD≤7.3%,平均回收率为92.73%~112.37%,RSD为3.2%~8.2%（n=6）,平均基质效应为90.33%~105.78%,RSD为3.2%~8.0%（n=6）。结论：该方法快速、灵敏,专属性强,可用于同时测定胎盘灌流液中双黄连冻干粉中8种成分的含量。OBJECTIVE： To establish a method for the simultaneous determination of the contents of Shuanghuanglian freeze-dried powder in placental perfusate. METHODS： HPLC was performed on the column of Agilent Zorbax-C18 with mobile phase of acetonitrile-1% formic acid aqueous solution （gradient elution） at flow rate of 1.0 ml/min, the internal standard was puera- rin, detection wavelength was 280 nm, column temperature was 25 ℃ and sample volume was 10 μl. RESULTS： There was a lin- ear range between linear ranges and peak area of 8 ingredients （r≥0.999 0）; RSDs of within-day and inter-day precision tests were no more than 1.9% , repeatability tests was no more than 7.3% ; average recoveries were in the range of 92.73% -112.37% （RSD-3.2%-8.2% ,n=6） ; and average matrix effects were 90.33%-105.78% （RSD=3.2%-8.0% ,n=6）. CONCLUSIONS： The method is rapid, sensitive and specific, and can be used to the simultaneous determination of the contents of 8 ingredients of Shuan- ghuanglian freeze-dried powder in placental perfusate.

关 键 词：双黄连冻干粉 高效液相色谱法 胎盘灌流液 绿原酸 连翘酯苷B 连翘酯苷A 黄芩苷 连翘苷 木犀草素 汉黄芩苷 黄芩素 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]