一株芽孢杆菌的分离、鉴定及其对仿刺参免疫的影响  

Isolation,Identification and Effect of a BacillusStrain on Immunity in Sea Cucumber Apostichopus japonicus

作　　者：关晓燕[1] 陈仲[1] 赵春霞 高杉[1] 蒋经伟[1] 王摆[1] 姜冰[1] 李丽丽[1,2] 顾英[3] 董颖[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁大连116023 [2]沈阳市农业行政执法支队,辽宁沈阳110014 [3]辽宁医学院食品科学与工程学院,辽宁锦州121000

出　　处：《水产科学》2015年第9期565-570,共6页Fisheries Science

基　　金：国家海洋公益项目(201305001);辽宁省海洋渔业厅科研项目(201301);辽宁科技计划项目(2014203006)

摘　　要：从大连湾地区健康的刺参养殖池塘沉积物中分离出一株优势菌株B4,通过菌落特征、生理生化特性和16SrDNA同源性分析,确定菌株B4为芽孢杆菌属。该菌株在温度20~35℃,pH 6~9,盐度3~7.5下生长良好。采用108cfu/mL密度的B4菌液投喂仿刺参,投喂后第0、7、14、21d和第28d测定仿刺参体腔液中酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶、溶菌酶和酚氧化酶的活性。试验结果表明,第7d时,试验组仿刺参腔液中碱性磷酸酶、超氧化物歧化酶和酚氧化酶的活性显著高于对照组（P〈0.05）;第21d时,试验组仿刺参腔液中溶菌酶的活性显著高于对照组;第28d时,酸性磷酸酶的活性与对照组具有极显著差异（P〈0.01）,说明B4可提高仿刺参体腔液中酸性磷酸酶、碱性磷酸酶、超氧化物歧化酶、溶菌酶和酚氧化酶5种免疫酶的活性,对仿刺参有一定的免疫刺激作用。A dominant strain B4 isolated from the sediment of a healthy sea cucumber Apostichopus japonicus farm in Dalian was identified as Bacillus by morphological and physiological characteristics and 16S rDNA sequence analysis. The optimal growth of strain B4 were observed at temperature of 20 35℃, pH of 6-9 and a salinity of 3 7.5. Then the activities of acid phosphatase （ACP）, alkaline phosphatase （AKP）, superoxide dismutase （SOD）, lysozyme （LSZ） and phenoloxidase （PO） were detected in the coelomic fluids of the sea cucumber exposed to the strain B4 at concentration of 108cfu/mL on 0, 7, 14, 21 and 28 day after administration. The results showed that there were significantly higher AKP, SOD and PO activities in the treatment groups than those in the control group 7 day after administration （P〈0.05）, while the coelomic fluid LSZ activity was higher at the 21th day （P〈0.05）. There was very significant difference in ACP activity on the 28th day between the control group and the test groups （P〈0.01）. The findings indicated that strain B4 led to improve the activities of AKP, ACP, PO, SOD and LSZ in coelomic fluid of sea cucumber, thus to have certain effect on immunity system.

关 键 词：芽孢杆菌 仿刺参 非特异性免疫酶活性. 

分 类 号：S968.9[农业科学—水产养殖]