电针对溃疡性结肠炎小鼠血清IL-6、TGF-β的影响  

IL-6、TGF-BETA Effect of the DSS Induced Ulcerative Colitis Mice Serum with Electroacupuncture

作　　者：耿煜[1] 孙军刚[1] 王程玉林 赵纪岚[1] 吴巧凤[1] 

机构地区：[1]成都中医药大学针灸推拿学院,四川成都610075

出　　处：《成都中医药大学学报》2015年第3期9-12,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81373737)

摘　　要：目的:观察电针对DSS诱导的UC模型小鼠的治疗效应及免疫因子IL-6、TGF-β的影响,初步探索电针治疗UC小鼠的免疫机制,为临床针灸治疗UC提供实验依据。方法:将38只昆明小鼠随机分为空白组、模型组及电针组。连续7 d饮用3%DSS溶液,诱发小鼠UC疾病发生。从造模第5 d开始取"足三里"、"关元"穴对电针组进行治疗,连续治疗5 d。各组小鼠记录疾病活动指数(DAI)、光学显微镜及透射电镜观察结肠组织形态,ELISA法检测血清中IL-6、TGF-β含量。结果:模型组DAI评分明显升高,电针组则显著下降(P<0.05)。结肠组织形态显示,光镜下模型组结肠黏膜组织结构不完整,腺体消失,杯状细胞增多,溃疡面及坏死灶形成,大量炎细胞浸润;电针组部分腺体受破坏,黏膜轻度水肿,少量炎细胞浸润。电镜下模型组微绒毛少而短、细胞内大量空泡,线粒体肿胀;电针组微绒较完整,线粒体肿胀减轻;空白组结肠组织形态正常。血清IL-6、TGF-β含量模型组均高于空白组(P<0.05),电针治疗组血清IL-6、TGF-β均下降低于模型组(P<0.05)。结论:电针治疗可能通过降低血清IL-6含量,抑制其与TGF-β协同作用从而减轻炎症反应,促进肠粘膜修复,以缓解UC引起腹泻便血等症状,发挥对UC的治疗作用。Objective： Study on the treatment effect of electric acupuncture （EA） and the influence of EA on IL-6 and TGF-13 with Ulcerative Colitis （UC） mice model induced by DSS. Preliminary explore the immune mechanism of EA treatment of UC mice, to provide experimental basis for clinical acupuncture treatment of UC. Methods： 38 KM mice were randomly divided into control group, model group and EA group. 3% DSS induced UC model mice, EA treatment group choose ＂Zu San Li, Guan yuan＂ points treated with EA from the fifth day after making model. Recording DAI, observed colon tissue morphology by optical microscope and transmission e- lectron microscope, ELISA assay the content of IL-6, TGF-[3 in serum in mice of each group. Results： The model group DAI score increased significantly, EA group decreased significantly （P 〈 0.05） . Under the light microscope, colonic mucosal tissue of model group structural incomplete, glands disappeared, goblet cells increased, ulcers and necrosis formation, a number of inflammatory cell infiltration. EA treatment group part of gland destruction, mucosa mild edema, a few inflammatory cells infiltration. Under electron microscope, model group microvilli are few and short, vacuoles in cells, mitochondrial swelling. After treatment, micro pile is more complete, mitochondrial swelling ease. Control group were no pathological changes under the light microscope and electron microscope. Serum IL-6, TGF-13 content of model group were higher than control group （ P 〈 0. 05 ） ; levels of IL-6 and TGF-13 were de- creased in EA treatment group compared with model group （ P 〈 0.05 ） . Conclusion： EA treatment may be through decreasing the content of IL-6 in serum, to inhibited TGF-13 synergistic effect to reduce inflammation, to promote the repair of intestinal mucosa, development of remission of UC disease, and play a role in the treatment of UC.

关 键 词：溃疡性结肠炎 电针 炎症因子 

分 类 号：R245[医药卫生—针灸推拿学] R392[医药卫生—中医临床基础]