变异白色红花总黄酮提取工艺研究  

Study on Extraction Technology of Total Flavones from White Safflower Variety

作　　者：周海玉[1] 赵春艳[1] 孙佩[2] 童文[2] 叶霄[2] 杨晓[2] 

机构地区：[1]成都中医药大学,四川成都611137 [2]四川省农业科学院经济作物育种栽培研究所,四川成都610300

出　　处：《成都中医药大学学报》2015年第3期27-31,共5页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省"十二五"农作物及畜禽育种攻关-特色种质资源收集评价与新材料和新方法创制项目(编号:2011NZ0098-12-01);四川省"十二五"财政(基因)育种工程-红花;赶黄草等分子标记辅助培育新品种项目(编号:2011JYGC02-006);四川省财政创新能力提升工程项目(青年基金)(编号:2013QNJJ-004)

摘　　要：目的:对变异白色红花总黄酮的提取工艺进行优化。方法:在单因素实验的基础上,以总黄酮含量为指标,采用正交实验设计考察渗漉法和回流法最佳工艺。结果:确定回流法提取效果优于渗滤法,回流提取的最佳工艺参数为:乙醇浓度60%,料液比1:15,回流提取2次,每次30 min,在此条件下白花提取液总黄酮含量达到18.51%。结论:回流提取工艺条件简单、稳定、可行,为变异白色红花黄酮的提取提供依据。Objective： To optimize the extraction technology of total flavones from White safflower variety. Methods： Based on single factor experiment, percolation method and ethanol reflux method were investigated by orthogonal design. The total flavones con- tent was considered as the indicator to optimize the main influencing factors. Results： The effect of reflux extraction was better than the percolation. The optimum reflux condition was using fifteen times the amount of 60% ethanol, extracting 2 times, 30 minutes for each time , by which the total flavones content of white safflower variety extraction reached 18.51%. Conclusion： The reflux extraction process is simple, stable and efficient. It can provide basis for extracting total flavones from white safflower variety .

关 键 词：变异白色红花 总黄酮 提取工艺 

分 类 号：R284.2[医药卫生—中药学] R285[医药卫生—中医学]