浦东地区部分猪场猪链球菌2型的分离鉴定及耐药性分析  

Identification and Drug Resistance Analysis of Streptococcus suis Type 2 from the Pudong Area

作　　者：韩少鹏[1] 俞向前 叶承荣 朱建国[1] 

机构地区：[1]上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海200240 [2]浦东新区动物疫病预防控制中心,上海201299

出　　处：《上海交通大学学报（农业科学版）》2015年第4期71-76,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：浦东新区科技发展基金创新资金(PKJ2014-N07);上海市生猪产业技术体系建设项目资助

摘　　要：2014年03月至08月期间，对来自浦东地区患关节炎的150份病猪和死猪内脏器官及关节液进行细菌分离，对分离细菌进行革兰氏染色、PCR鉴定、药敏实验和多重PCR检测链球菌毒力因子分布（荚膜多糖cps、溶菌酶释放蛋白基Nmrp、胞外因子ef、溶血素sly、毒力相关基因orf2、谷氨酸脱氢酶基因gdh、甘油醛-3-磷酸脱氢酶基因gapdh），并对链球菌2型cps基因序列进行进化树分析。结果，从分离到的120株细菌中检测到21株链球菌，分离率为17．5％，2型链球菌分离率为11．67％；链球菌的毒力型呈-／＋cps2J-／＋优修Pf-sly-gdh-／＋gadph-／＋orf2；分离株呈多重耐药性，耐药性为：大环内酯类〈硝基呋喃类、氯霉素类、多肽类、氨基糖苷类、四环素类〈青霉素类、头孢菌素类、亏诺酮类〈林可胺类、磺胺类；cps基因与GenBank上已公布的链球菌2型荚膜多糖基因的核苷酸同源性达94％～99％。From Mar. to Aug. 2014, sick or dead pigs affected with arthrophlogosis from the Pudong area were sampled. The 120 strains of bacteria, isolated from 150 internal organs and joint fluid, were tested by gram stain and multiple PCR methods of detecting virulence factors which include genes about mrp, e f, sly,orf2,gdh and fbps. In addition, the sequence of cps gene of Streptococcus suis type 2 were compared with those of GcnBank and a susceptibility testing of isolates were taken. The results showed that the virulence type of 21 strains of Streptococcus （17. 5%） and 14 strains of Streptococcus suis type 2 （11.6%） was -/＋cps2J-/4-mrp-ef-sly-gdh/-4-gadph-/-4-off2. The 21 strains of Streptococcus showed multi-drug resistance and the strength of drug resistance indicated the following results：Macrolides Nitrofurans,Fenicols, Methylmercadone, Aminoglycosides, Tetracyclines 〈 Penicillins, Cephalosporins, Quinolones I.incosamide,Sulfonamides. The identification homology of capsular polysaccharide gene of Streptococcus suis type 2 was 94～99%.

关 键 词：关节炎 猪链球菌2型 毒力型 耐药性 

分 类 号：S852.611[农业科学—基础兽医学]