MiRNA-1247-3P在急性呼吸窘迫综合征细胞模型中的表达及功能分析  

Expression of mi RNA-1247-3P in acute respiratory distress syndrome cell model and its function

作　　者：程东良[1] 梁媛 陈衍晨[3] 卿娣 史长松[1] 

机构地区：[1]郑州大学人民医院儿科(河南省人民医院儿科),450000 [2]河南省开封市儿童医院,470000 [3]南方医科大学珠江医院儿科,广州市510282 [4]广州市妇女儿童医疗中心,510623

出　　处：《实用医学杂志》2015年第17期2802-2804,共3页The Journal of Practical Medicine

摘　　要：目的:检测脂多糖(LPS)处理A549细胞前后发生显著变化的mi RNAs,并验证mi R-1247-3P在不同浓度LPS处理后细胞中表达量的变化,并探讨其可能机制。方法:对照组是以培养液处理A549细胞,实验组分别以不同浓度的LPS处理A549细胞。通过免疫细胞化学染色法、RT-PCR方法检测各组细胞中的SP-A、SP-C的表达量。用基因芯片检测细胞处理前后的表达谱,找出其中的关键mi RNA,PCR的方法检测各组细胞中mi RNA的表达量。结果:实验组中各组细胞的SP-A、SP-C的表达量较对照组均下降(P<0.05)。mi RNAs芯片检测结果显示:表达显著上调的有31个,显著下调的有3个。实时荧光定量PCR测出各实验组mi RNA-1247-3P的相对表达量较对照组均上升(P<0.05)。结论:mi RNA-1247-3P在实验组有高表达,提示其可能在ARDS的发生发展过程中发挥作用。Objective To detect the expression pattern of microRNA in A549 cells treated by lipopolysaccharide, study the expression of miRNA-1247-3P in A549 cells under LPS treatment and explore the possible mechanism of miRNA-1247-3P in A549 cells under LPS treatment. Methods A549 cells were divided into experimental and control groups. [mmunocytochemical method and RT-PCR were used to detect the changes of SP-A and SP-C. The expression of miRNAs were detected using miRNAs array in different groups. The key miR-1247-3P was collected to detect the changes of miR-1247-3P in all groups using quantitative real-time polymerase chain reaction. Results Compared with control group, the expressions of SP-A and SP-C weresignificantly decreased in the experimental groups （P 〈 0.05）. MiRNA array showed that 51 miRNAs were up- regulated and 3 miRNAs were down-regulated. Compared with control group, the expression of miR-1247-SP was significantly increased in the experimental groups （P 〈 0.05）. Conclusion The increased expression of miR- 1247-3P may play an important role in the pathogenesis of ARDS.

关 键 词：急性呼吸窘迫综合征 microRNA-1247-3P 肺表面活性蛋白A A549细胞 

分 类 号：R563.8[医药卫生—呼吸系统]