高致病性猪繁殖与呼吸综合征GD强弱毒株感染对猪外周血淋巴细胞亚群的影响  

Study on Changes of Lymphocyte Subpopulations in Peripheral Blood of the Pigs Inoculated with Highly Pathogenic PRRSV GD Wild and Vaccine Strain

作　　者：张文利[1] 王海光[1] 孙丰廷[1,2] 刘灿[3] 英聪[1,2] 宁宜宝[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]华中农业大学,武汉430070 [3]中国农业大学,北京100193

出　　处：《中国兽药杂志》2015年第9期1-6,共6页Chinese Journal of Veterinary Drug

摘　　要：为了从细胞水平探讨PRRSV GD强毒株和PRRSV弱毒疫苗接种仔猪后对其外周血淋巴细胞亚群的影响,运用血常规技术和流式细胞技术分析了猪体外周血淋巴细胞亚群的动态变化。将30日龄20头SPF猪分成3组,分别感染HP-PRRSV GD第5代强毒株、GDr180弱毒疫苗株及留作对照。于感染后第0、3、7、10、14、21、28及35天采血进行外周血淋巴细胞亚群测定,分析淋巴细胞亚群的变化。结果表明:HP-PRRSV GD强毒株感染可导致白细胞、淋巴细胞数量、单核细胞、粒细胞、B细胞、Tc细胞、Th细胞、Tm细胞、和γδT细胞数量的下降;而弱毒疫苗GDr180株免疫则表现为白细胞、淋巴细胞、单核细胞、引起粒细胞、B细胞、Tc细胞、Th细胞和Tm细胞的上升,对γδT细胞的影响不大;阴性对照组在整个检测期间各种细胞基本上保持稳定状态。从实验结果可以看出:与HP-PRRSV GD强毒株破坏免疫细胞不同,PRRSV弱毒疫苗GDr180株免疫接种能刺激猪免疫细胞增殖,给试验猪提供良好的免疫反应。In the study, we used an immunofluorescence assay to investigate changes in lymphocyte subpopulations in peripheral blood of the specific-pathogen-free（ SPF） pigs inoculated with highly pathogenic porcine reproductive and respiratory syndrome virus（ HP-PRRSV） GD wild and vaccine strains,which was in a cellular way to discuss the effect between the lymphocyte subpopulations in peripheral blood of the SPF pigs and HP-PRRSV GD wild and vaccine type. SPF pigs inoculated with PRRS wild virus showed remarkable decreases in total lymphocytes,white blood cells,monocytes,granulocytes,B cells,Tc cells,Th cells,Tm cells and γδT cells,while SPF pigs inoculated with PRRS vaccine virus increases in total lymphocytes,white blood cells,monocytes,granulocytes,B cells,Tc cells,Th cells,Tm cells after PID 3,and the numbers of all cells remain substantially stable in the negative control group. From these results we can conclude that HP-PRRSV GD wild type can destroy immune cells,while HP-PRRSV GD vaccine type can stimulate immune cell proliferation,which provides a good immune response to the pigs.

关 键 词：高致病性猪繁殖与呼吸综合征 疫苗株 野毒株 猪 外周血淋巴细胞亚群 

分 类 号：S858.28[农业科学—临床兽医学]