基于双柱固相萃取-色质联用技术同时检测鸡肉中8种抗病毒药物残留  

Simultaneous Analysis of Eight Antiviral Drug Residues in Chicken Based on a Double-column Designed Solid Phase Extraction Combined with LC-MS/MS Method

作　　者：张鑫[1] 吴剑平[1] 严凤[1] 陆淳[1] 顾欣[1] 

机构地区：[1]上海市动物疫病预防控制中心,上海201103

出　　处：《中国兽药杂志》2015年第9期45-50,共6页Chinese Journal of Veterinary Drug

基　　金：上海市市级农口系统青年人才成长计划:沪农青字(2014)第2-5号;公益性行业(农业)科研专项(201203023)

摘　　要：建立了双柱固相萃取-液相色谱-串联质谱法,可实现对鸡肉中金刚烷胺、金刚乙胺、美金刚胺、吗啉胍、利巴韦林、阿昔洛韦、更昔洛韦、奥司他韦等8种抗病毒药物的同时分析。采用三氯乙酸水溶液-乙腈对鸡肉中8种抗病毒药物残留同时提取,依次通过石墨化碳柱和强阳离子交换柱以实现对目标药物的富集、净化,选用石墨化碳色谱柱进行分离,超高效液相色谱-串联质谱检测。本文通过研究不同提取溶液、固相萃取柱及色谱柱对检测结果的影响,最终建立优化的检测方法,采用外标法计算,检测限1μg/kg,定量限2μg/kg,添加浓度在2~200μg/kg范围内的回收率大于80%,适用于鸡肉中8种抗病毒药物的同时分析。A novel detection method based on a double-column designed solid phase extraction（ SPE） combined with LC-MS / MS was developed for the simultaneous analysis of eight antiviral drugs residues in chicken,including amantadine,rimantadine,memantine,moroxydine,ribavirin,acyclovir,ganciclovir and oseltamivir. A graphitic carbon column and a strong cation exchange column were connected and used in series to enrich and purify the analytes from the trichloroacetic acid-acetonitrile aqueous solution. The eight drugs were separated by Hypercarb column and detected by LC-MS / MS method. The influences of the used dilutionsolvent,solid phase extractioncolumns andchromatographic columns on the test results were investigated to optimize the detection method. The results showed a low limit of detection down to 1 μg / kg. And the limit of quantization of 2 μg / kg was achieved. Moreover,the fortified concentrations could range from 2 to 200 μg / kg,and the recovery was higher than 80%. Hence,the detection method presented would be a promising candidate if the quick analysis for multicomponent and complicated samples was needed.

关 键 词：石墨化碳 抗病毒药物 残留检测 

分 类 号：S859.84[农业科学—临床兽医学]